CAJ | 학술논문

采用抗利福平标记菌株的平板菌落计数法，检测了内生解淀粉芽孢杆菌 Bacillus amyloliquefaciens CC09在小麦根部的定殖能力及消长动态，发现菌株CC09在小麦根内、根表和根际均能定殖，接种后5 d定殖量分别稳定维持在2.7×105、2.0×105和5.5×105 cfu/g。透射电镜观察发现，菌株CC09能够在小麦根组织的皮层细胞、细胞间隙、中柱鞘及髓腔中定殖，且不影响根组织细胞结构。室内盆栽试验表明，用菌株CC09发酵液灌根处理小麦，对由禾谷镰刀菌Fusarium graminearum侵染引起的麦苗赤霉病的防效高达90.7%，表明菌株CC09是潜在的防治小麦赤霉病的生防菌，具有良好的开发和应用价值。
채용항리복평표기균주적평판균락계수법，검측료내생해정분아포간균 Bacillus amyloliquefaciens CC09재소맥근부적정식능력급소장동태，발현균주CC09재소맥근내、근표화근제균능정식，접충후5 d정식량분별은정유지재2.7×105、2.0×105화5.5×105 cfu/g。투사전경관찰발현，균주CC09능구재소맥근조직적피층세포、세포간극、중주초급수강중정식，차불영향근조직세포결구。실내분재시험표명，용균주CC09발효액관근처리소맥，대유화곡렴도균Fusarium graminearum침염인기적맥묘적매병적방효고체90.7%，표명균주CC09시잠재적방치소맥적매병적생방균，구유량호적개발화응용개치。
Bacillus amyloliquefaciens can colonize many plant roots, promote plant growth and suppress pathogenic fungi. However, its colonization process and potential disease control efficacy on wheat roots and rhizosphere are rarely studied. By applying the rifampicin resistant mutant and plate diluting method, we have investigated the colonization capacity and population dynamics of endophytic B. amyloliquefaciens CC09 within wheat root tissues, surface and rhizosphere. Results showed that CC09 strain colonized well within root tissues, surfaces and rhizosphere with a density of 2.7×105, 2.0×105 and 5.5×105 cfu/g, respectively, at 5 d after inoculation, and maintained at these population levels thereafter. Moreover, the strain could colonize in the cortical cells, intercellular spaces, pericycle cells and canal tissues in wheat root tissues and did not show clear adverse impacts on the cell ultra-structures based on the transmission electron microscopy (TEM) observation. In addition, the cultural broth of the strain exhibited 90.7% control efficiency against wheat seedling blight caused by Fusarium graminearum using root-drenching pot assay under the greenhouse conditions. These results suggested that the endophytic B. amyloliquefaciens CC09 has the potential to be developed as biocontrol agent to control wheat disease caused by the pathogenic F. graminearum.