CAJ | 학술논문

对昆虫病原体的直接取样、检测及定量化能够直接反映昆虫流行病学调查中的病原体丰度。本文以在田间蚜群中广泛流行的虫霉目真菌新蚜虫疠霉为研究对象，通过对不同来源的新蚜虫疠霉样品的 DNA提取测试，建立Lysis提取结合Chelex-100方法的PCR检测技术体系，并以此方法为支撑进行了田间麦蚜新蚜虫疠霉流行病的调查验证。结果表明改良的Chelex方法不仅为昆虫病原真菌的田间流行学调查提供了一个快速、准确的评价方法，而且在其他真菌的核酸快速、痕量检测方面也可能具有重要的应用潜力。
대곤충병원체적직접취양、검측급정양화능구직접반영곤충류행병학조사중적병원체봉도。본문이재전간아군중엄범류행적충매목진균신아충려매위연구대상，통과대불동래원적신아충려매양품적 DNA제취측시，건립Lysis제취결합Chelex-100방법적PCR검측기술체계，병이차방법위지탱진행료전간맥아신아충려매류행병적조사험증。결과표명개량적Chelex방법불부위곤충병원진균적전간류행학조사제공료일개쾌속、준학적평개방법，이차재기타진균적핵산쾌속、흔량검측방면야가능구유중요적응용잠력。
Direct sampling, detection and quantification of entomopathogens can directly reflect the epizootiology of pest in abundance. Pandora neoaphidis, a widespread obligated-aphid pathogen, was investigated in aphid populations. A PCR technology system based on modified Lysis extraction associated with Chelex-100 was developed for epizootiological investigation of entomophthoralean fungi on wheat aphid. Results show that the developed method not only provide a rapid and accurate tool for epizootiological investigation of entomopathogenic fungus, but also have a potential application for rapid and tiny-nucleic acid detection in other fungus.