CAJ | 학술논문

目的：：建立金莲花口服液有效的质量评价方法。方法：通过建立HPLC指纹图谱和检测金莲花口服液中有效成分荭草苷、牡荆素的含量，对市售金莲花口服液的质量进行分析。采用Phenomenex C18(4.6×250mm,5μm)色谱柱；乙腈-0.1%磷酸-0.1%四氢呋喃溶剂系统梯度洗脱；检测波长230nm；流速1.0ml/min。结果：确定了20个共有峰，市售金莲花口服液指纹图谱峰相似度均＞0.94；荭草苷的线性范围：10.00-392.80μg/ml(r=0.9999)，牡荆苷的线性范围：2.40-94.90μg/ml(r=0.9999)；荭草苷平均回收率为101.85%，RSD为1.61%(n=6)，牡荆苷平均回收率为102.53%，RSD为1.45%(n=6)。结论：本研究建立的金莲花口服液指纹图谱，以及荭草苷和牡荆素含量测定方法简便、准确、灵敏度高，稳定性和重现性良好，可为金莲花口服液的质量控制提供依据。
목적：：건립금연화구복액유효적질량평개방법。방법：통과건립HPLC지문도보화검측금연화구복액중유효성분홍초감、모형소적함량，대시수금연화구복액적질량진행분석。채용Phenomenex C18(4.6×250mm,5μm)색보주；을정-0.1%린산-0.1%사경부남용제계통제도세탈；검측파장230nm；류속1.0ml/min。결과：학정료20개공유봉，시수금연화구복액지문도보봉상사도균＞0.94；홍초감적선성범위：10.00-392.80μg/ml(r=0.9999)，모형감적선성범위：2.40-94.90μg/ml(r=0.9999)；홍초감평균회수솔위101.85%，RSD위1.61%(n=6)，모형감평균회수솔위102.53%，RSD위1.45%(n=6)。결론：본연구건립적금연화구복액지문도보，이급홍초감화모형소함량측정방법간편、준학、령민도고，은정성화중현성량호，가위금연화구복액적질량공제제공의거。
Objective: To establish effective quality evaluation methods ofTrollii FlosOral Liquid (TFOL).Methods:The quality of commercially available TFOL was analyzed by establishing ifngerprint by HPLC and determining the content of active ingredients, orientin and vitexin. Phenomenex C18column (4.6×250mm, 5μm) was used with mobile phase was acetonitrile-0.1% phosphoric acid-0.1% tetrahydrofuran, determine wavelength was 230nm, lfow velocity was 1.0ml/min. Results:This study determined 20 common peaks, and the similarity ifngerprint peaks of commercially available TFOL were all greater than 0.94. The linear range of orientin and vitexin were 10.00-392.80(r= 0.9999) and 2.40-94.90 μg/ml (r = 0.9999); the average recovery rate were 101.85% and 102.53%; the RSD were 1.61% (n=6) and 1.45% (n=6).Conclusions:The method in this study that establishing TFOL ifnger print and determination of orientin and vitexin is simple, accurate, high sensitive, and also with better stability and reproducibility. So this method can provide basis for quality control of TFOL.