承德医学院学报
承德醫學院學報
승덕의학원학보
JOURNAL OF CHENGDE MEDICAL
2014年
6期
461-463
,共3页
徐杨璐%赵胜男%申川川%李守拙
徐楊璐%趙勝男%申川川%李守拙
서양로%조성남%신천천%리수졸
黄芩%高效液相色谱-二极管阵列检测器-质谱法%质量控制
黃芩%高效液相色譜-二極管陣列檢測器-質譜法%質量控製
황금%고효액상색보-이겁관진렬검측기-질보법%질량공제
Scutellaria baicalensis Georgi%HPLC-DAD-MS%Quality control
目的：：分析比较高效液相色谱-二极管阵列检测器-质谱(HPLC-DAD-MS)法和高效液相色谱-二极管阵列检测器(HPLC-DAD)法检测黄芩中3种黄酮类化合物的结果。方法：分别采用HPLC-DAD-MS法和HPLC-DAD法检测黄芩中3种黄酮类化合物黄芩苷、汉黄芩苷、汉黄芩素的含量。结果：HPLC-DAD-MS法测得黄芩苷、汉黄芩苷、汉黄芩素的含量分别比HPLC-DAD法平均少6.61%、73.98%和10.90%，其中汉黄芩苷的含量相差较大。结论：与HPLC-DAD法相比，HPLC-DAD-MS法含量测定结果更准确，且操作简便、重现性好，可用于黄芩中黄芩苷、汉黄芩苷、汉黄芩素的含量测定。
目的：：分析比較高效液相色譜-二極管陣列檢測器-質譜(HPLC-DAD-MS)法和高效液相色譜-二極管陣列檢測器(HPLC-DAD)法檢測黃芩中3種黃酮類化閤物的結果。方法：分彆採用HPLC-DAD-MS法和HPLC-DAD法檢測黃芩中3種黃酮類化閤物黃芩苷、漢黃芩苷、漢黃芩素的含量。結果：HPLC-DAD-MS法測得黃芩苷、漢黃芩苷、漢黃芩素的含量分彆比HPLC-DAD法平均少6.61%、73.98%和10.90%，其中漢黃芩苷的含量相差較大。結論：與HPLC-DAD法相比，HPLC-DAD-MS法含量測定結果更準確，且操作簡便、重現性好，可用于黃芩中黃芩苷、漢黃芩苷、漢黃芩素的含量測定。
목적：：분석비교고효액상색보-이겁관진렬검측기-질보(HPLC-DAD-MS)법화고효액상색보-이겁관진렬검측기(HPLC-DAD)법검측황금중3충황동류화합물적결과。방법：분별채용HPLC-DAD-MS법화HPLC-DAD법검측황금중3충황동류화합물황금감、한황금감、한황금소적함량。결과：HPLC-DAD-MS법측득황금감、한황금감、한황금소적함량분별비HPLC-DAD법평균소6.61%、73.98%화10.90%，기중한황금감적함량상차교대。결론：여HPLC-DAD법상비，HPLC-DAD-MS법함량측정결과경준학，차조작간편、중현성호，가용우황금중황금감、한황금감、한황금소적함량측정。
Objective:To compare the determination of three lfavonoids inScutellaria baicalensis Georgi by high performance liquid chromatography with diode array detection and mass spectrometer (HPLC-DAD-MS) and high performance liquid chromatography with diode array detection (HPLC-DAD).Methods: HPLC-DAD-MS and HPLC-DAD were respectively applied to determineBaicalin,Wogonoside and Wogonin inScutellaria baicalensis Georgi. Results:The content ofBaicalin, Wogonoside, Wogonin by HPLC-DAD-MS were 6.61%, 73.98% and 10.90% less than HPLC-DAD. Among them, the difference ofWogonoside content was fairly larger.Conclusions: Compared with HPLC-DAD, HPLC-DAD-MS is more accurate, simple and convenient. So HPLC-DAD-MS can be used for the determination of Baicalin, Wogonoside, WogonininScutellaria baicalensis Georgi.
