CAJ | 학술논문

目的：检测宫颈癌组织中人乳头瘤病毒（ HPV）感染率及人半翼基因（ HWAPL）蛋白表达水平，探讨高危型HPV感染与HWAPL蛋白表达的关系。方法：收集宫颈上皮内瘤变（ CIN）组织及宫颈癌组织石蜡标本共132例，其中CINⅠ29例、CINⅡ28例、CINⅢ35例、宫颈癌40例，应用HPV分型基因芯片系统检测其内HPV并分型，应用PV-9000法进行HWAPL染色。结果：宫颈癌和CINⅢ组织中高危型HPV阳性率高于CINⅡ和CINⅠ组织（χ2＝34．560，P＜0．001）。宫颈癌和 CIN 组织中 HWAPL 蛋白表达强度比较，差异有统计学意义（H＝58．981，P ＜0．001）。高危型HPV感染与HWAPL蛋白表达有关联（rP ＝0．356，P＝0．001）。结论：HWAPL蛋白异常表达可能是高危型HPV致宫颈癌发病的重要环节。
목적：검측궁경암조직중인유두류병독（ HPV）감염솔급인반익기인（ HWAPL）단백표체수평，탐토고위형HPV감염여HWAPL단백표체적관계。방법：수집궁경상피내류변（ CIN）조직급궁경암조직석사표본공132례，기중CINⅠ29례、CINⅡ28례、CINⅢ35례、궁경암40례，응용HPV분형기인심편계통검측기내HPV병분형，응용PV-9000법진행HWAPL염색。결과：궁경암화CINⅢ조직중고위형HPV양성솔고우CINⅡ화CINⅠ조직（χ2＝34．560，P＜0．001）。궁경암화 CIN 조직중 HWAPL 단백표체강도비교，차이유통계학의의（H＝58．981，P ＜0．001）。고위형HPV감염여HWAPL단백표체유관련（rP ＝0．356，P＝0．001）。결론：HWAPL단백이상표체가능시고위형HPV치궁경암발병적중요배절。
Aim:To detect the infection rate of high-risk HPV and the expression level of HWAPL in cervical cancer tissue and to investigate the relationship between them .Methods:Gene chip technology was used to detect HPV genoty-ping,at the same time PV-9000 immunohistochemistry was used to detect HWAPL protein expression in 132 specimens(in-cluding CINⅠ29 cases, CINⅡ28 cases, CINⅢ35 cases, and cervical cancer 40 cases).Results:The positive rates of high-risk HPV in cervical cancer and CINⅢwere significantly higher than those in CINⅡ and CINⅠ(χ2 =34.560,P<0.001).The expression level of HWAPL protein in cervical cancer and CIN were significantly different (H=58.981,P<0.001).High-risk HPV infection was positively correlated with HWAPL protein expression (rP =0.356,P =0.001). Conclusion:The abnormal expression of HWAPL may be an important part for high-risk HPV induced cervical cancer .