CAJ | 학술논문

目的：探讨人脐带沃顿胶干细胞（ WJCs ）移植对急性脊髓损伤大鼠的治疗作用及其可能机制。方法：81只大鼠分为假手术组、模型组和WJCs移植组，每组27只。每组取6只，采用BBB评分法评估脊髓损伤后1、3、7、14、21和28 d各组大鼠的运动功能，采用透射电镜检测损伤局部超微结构并检测损伤后28 d脑源性神经营养因子（ BDNF）的表达，其余大鼠采用实时荧光定量PCR和Western blot检测损伤后0、3、6、12、24、72、168 h损伤脊髓组织中肿瘤坏死因子（ TNF-α）的表达。结果：脊髓损伤后模型组与WJCs移植组大鼠的运动功能均有不同程度的恢复，其中WJCs移植组较模型组恢复更明显。与模型组相比，WJCs移植组损伤区脊髓组织结构相对完整。模型组和WJCs移植组损伤脊髓组织中TNF-αmRNA和蛋白以及BDNF蛋白的表达较假手术组增加（P＜0．05）；与模型组相比，WJCs移植组中TNF-α的表达降低，而BDNF的表达增加（ P＜0．05）。结论：WJCs移植可改变脊髓损伤区的微环境，抑制损伤脊髓组织中TNF-α的表达，同时增加BDNF的表达，促进大鼠神经功能恢复，减少脊髓继发性损伤。
목적：탐토인제대옥돈효간세포（ WJCs ）이식대급성척수손상대서적치료작용급기가능궤제。방법：81지대서분위가수술조、모형조화WJCs이식조，매조27지。매조취6지，채용BBB평분법평고척수손상후1、3、7、14、21화28 d각조대서적운동공능，채용투사전경검측손상국부초미결구병검측손상후28 d뇌원성신경영양인자（ BDNF）적표체，기여대서채용실시형광정량PCR화Western blot검측손상후0、3、6、12、24、72、168 h손상척수조직중종류배사인자（ TNF-α）적표체。결과：척수손상후모형조여WJCs이식조대서적운동공능균유불동정도적회복，기중WJCs이식조교모형조회복경명현。여모형조상비，WJCs이식조손상구척수조직결구상대완정。모형조화WJCs이식조손상척수조직중TNF-αmRNA화단백이급BDNF단백적표체교가수술조증가（P＜0．05）；여모형조상비，WJCs이식조중TNF-α적표체강저，이BDNF적표체증가（ P＜0．05）。결론：WJCs이식가개변척수손상구적미배경，억제손상척수조직중TNF-α적표체，동시증가BDNF적표체，촉진대서신경공능회복，감소척수계발성손상。
Aim:To explore the effect of Wharton′s jelly cells (WJCs) transplant on the expressions of tumor necrosis factor alpha (TNF-α) and brain-derived neurotrophic factor (BDNF) in rats with acute spinal cord injury (SCI), and to in-vestigate the function of WJCs in the inflammatory response and nerve repair after acute SCI .Methods:A total of 81 rats were allocated into sham operation group , model group and WJCs group (27 rats in each group).In the WJCs group, 200μL WJCs cell suspension (about 1 ×106 cells) was injected by femoral vein at 10 min after the injury.Neurologic function was accessed by BBB score at 1 d, 3 d, 7 d, 14 d, 21 d and 28 d respectively after transplantation;the ultrastructures of injured spinal cord were detected by transmission electron microscopy;the mRNA and protein expressions of BDNF in the spinal cord tissue were detected by real-time quantitative PCR and Western blot , respectively at 28 d, and those of TNF-αlevel were detected at 0 h, 3 h,6 h, 12 h, 24 h, 72 h, 168 h after transplantation .Results:Neural function was recov-ered to some extent both in WJCs group and model group , and the former was much better .Compared with model group , the structure of SCI area of WJCs group was relatively intact .Compared with the sham operation group , the expressions of BDNF protein and TNF-αmRNA and protein were increased significantly both in model group and WJCs group (P<0.05);compared with model group, the expressions of TNF-α mRNA and protein decreased(P<0.05), while that of BDNF in-creased in the WJCs group(P<0.05) .Conclusion:WJCs transplantation can change the microenvironment of the injured spinal cord by inhibiting the expression of TNF-αwhile promoting the expression of BDNF in the lesion , therefore reducing secondary spinal cord injury , which may be a potential mechanism underlying the recovery of motor function of injured rats .