CAJ | 학술논문

目的：研究富氢水对糖尿病小鼠肾脏的保护作用，并探讨其可能的作用机制。方法：60只健康ICR雄性小鼠，随机分成空白对照组（A组）、糖尿病组（B组）和糖尿病富氢水干预组（C组）。链脲佐菌素（STZ）150mg/kg腹腔注射诱发糖尿病，造模成功后，C组每日腹腔注射富氢水5mL/kg，B组腹腔注射等量0.9%氯化钠溶液，连续8周。尾静脉取血测定小鼠血糖，HE染色观察肾脏组织学变化，免疫组织化学染色观察NADPH氧化酶4（Nox4）和血红素加氧酶-1（HO-1）蛋白的表达。结果：C组Nox4表达水平明显低于B组，而HO-1表达明显高于B组；B组与C组的空腹血糖均明显高于A组（P＜0.01），B组与C组之间差异无统计学意义（P＞0.05）；3组肾脏组织病理HE染色未显示明显病变。结论：富氢水可减轻糖尿病小鼠肾脏组织的氧化应激水平。
목적：연구부경수대당뇨병소서신장적보호작용，병탐토기가능적작용궤제。방법：60지건강ICR웅성소서，수궤분성공백대조조（A조）、당뇨병조（B조）화당뇨병부경수간예조（C조）。련뇨좌균소（STZ）150mg/kg복강주사유발당뇨병，조모성공후，C조매일복강주사부경수5mL/kg，B조복강주사등량0.9%록화납용액，련속8주。미정맥취혈측정소서혈당，HE염색관찰신장조직학변화，면역조직화학염색관찰NADPH양화매4（Nox4）화혈홍소가양매-1（HO-1）단백적표체。결과：C조Nox4표체수평명현저우B조，이HO-1표체명현고우B조；B조여C조적공복혈당균명현고우A조（P＜0.01），B조여C조지간차이무통계학의의（P＞0.05）；3조신장조직병리HE염색미현시명현병변。결론：부경수가감경당뇨병소서신장조직적양화응격수평。
Objective: To study the protective effect of hydrogen on renal injury induced by type 1 dibetes in mice and explore the possible mechanism.Methods: Sixty healthy male ICR mice were randomly divided into control group (A), diabetic group (B) and diabetes hydrogen water intervention group (C). Diabetes were induced by intraperitoneal injection of streptozotocin 150 mg/kg. Group C were injected saturated hydrogen-rich water 5 mL/kg and group B were injected with equal normal saline every day for 8 weeks in mice models of type 1 dibetes. The blood was collected from the tail vein and blood glucose was measured. Renal injury were identiifed by hematoxylin and eosin (HE) staining. Expression of Nox4 and HO-1 were identiifed by immuno-histochemical staining.Results: Expression of Nox4 in Group C was signiifcantly lower than that in group B, expression of HO-1 in Group C was signiifcantly higher than that in group B. Blood glucose in Group B and C were higher than that in Group A, but there was no difference between the two groups. Kidney HE staining did not show signiifcant lesions.Conclusion: Hydrogen-rich water can reduce oxidative stress in renal tissue of dia-betic mice.