CAJ | 학술논문

目的：观察同一胎鼠肠神经干细胞和脑神经干细胞的神经递质表达情况，探索神经干细胞移植治疗相关疾病的合适干细胞源。方法：从孕20d的同一SD胚鼠中分别提取肠神经干细胞和脑神经干细胞，采用改良的神经干细胞培养液反复传代，取传代培养6～10d后得到的2种不同来源的神经细胞球，通过琼脂糖预包埋免疫细胞化学检测法及流式细胞仪检测法测定神经干细胞4种神经递质的表达。结果：2种不同来源的神经干细胞的琼脂糖预包埋免疫细胞化学检测显示：肠神经干细胞神经元型一氧化氮合酶（nNOS）、胆碱乙酰转移酶（ChAT）与相应的对照组比较差异有统计学意义（P＜0.05）；脑神经干细胞nNOS、ChAT、多巴胺β羟化酶（DβH）与相应的对照组比较差异有统计学意义（P＜0.05）；2种不同来源神经干细胞间nNOS、ChAT比较差异有统计学意义（P＜0.05）。流式细胞仪检测提示：肠神经干细胞γ-氨基丁酸（GABA）、nNOS、ChAT、DβH阳性细胞合计率分别为1.59%、14.97%、5.43%、1.36%，其中nNOS、ChAT与相应的同型对照组比较差异有统计学意义（P＜0.05）；而脑神经干细胞GABA、nNOS、ChAT、DβH阳性细胞合计率分别为1.68%、6.14%、25.42%、9.30%，其中nNOS、ChAT、DβH与相应的同型对照组比较差异有统计学意义（P＜0.05）。结论：肠神经干细胞表达的神经递质主要为一氧化氮（NO）和乙酰胆碱（Ach），以NO为主；脑神经干细胞表达的神经递质为NO、Ach和去甲肾上腺素（NA），以Ach为主；2种神经干细胞均无明显表达GABA。
목적：관찰동일태서장신경간세포화뇌신경간세포적신경체질표체정황，탐색신경간세포이식치료상관질병적합괄간세포원。방법：종잉20d적동일SD배서중분별제취장신경간세포화뇌신경간세포，채용개량적신경간세포배양액반복전대，취전대배양6～10d후득도적2충불동래원적신경세포구，통과경지당예포매면역세포화학검측법급류식세포의검측법측정신경간세포4충신경체질적표체。결과：2충불동래원적신경간세포적경지당예포매면역세포화학검측현시：장신경간세포신경원형일양화담합매（nNOS）、담감을선전이매（ChAT）여상응적대조조비교차이유통계학의의（P＜0.05）；뇌신경간세포nNOS、ChAT、다파알β간화매（DβH）여상응적대조조비교차이유통계학의의（P＜0.05）；2충불동래원신경간세포간nNOS、ChAT비교차이유통계학의의（P＜0.05）。류식세포의검측제시：장신경간세포γ-안기정산（GABA）、nNOS、ChAT、DβH양성세포합계솔분별위1.59%、14.97%、5.43%、1.36%，기중nNOS、ChAT여상응적동형대조조비교차이유통계학의의（P＜0.05）；이뇌신경간세포GABA、nNOS、ChAT、DβH양성세포합계솔분별위1.68%、6.14%、25.42%、9.30%，기중nNOS、ChAT、DβH여상응적동형대조조비교차이유통계학의의（P＜0.05）。결론：장신경간세포표체적신경체질주요위일양화담（NO）화을선담감（Ach），이NO위주；뇌신경간세포표체적신경체질위NO、Ach화거갑신상선소（NA），이Ach위주；2충신경간세포균무명현표체GABA。
Objective: To observe the expression of neurotransmitters in enterogenous neural stem cells (ENSCs) and central nervous system neural stem cells (CNS-NSCs) from the same fetal rats, and to explore a suitable stem cell source for neural stem cells transplantation.Methods: Rat ENSCs and CNS-NSCs were ex-tracted from the same 20-day SD rat embryos followed by repeated subculture using improved neural stem cell medium. Two different kinds of nerve bulbs originated from 6-10 days culturing were chosen. The expressions of four transmitters on ENSCs and CNS-NSCs were analyzed by agarose pre-embedded immunocytochemistry as-say and lfow cytometry.Results: After analyzing data of imunocytochemistry assay, we found the expression of nNOS and ChAT on ENSCs; nNOS, ChAT and DβH on CNS-NSCs. Signiifcant differences of nNOS and ChAT expression were observed between ENSCs and CNS-NSCs respectively (P<0.05). Flow cytometry analysis in-dicated GABA(+), nNOS(+), ChAT(+), DβH(+) cells averagely accounted for 1.59%, 14.97%, 5.43%, 1.36% in ENSCs respectively,within which nNOS and ChAT expression were signiifcantly different from isotype groups (P<0.05). In CNS-NSCs, the pecentages were 1.68%, 6.14%, 25.42%, 9.30% on average, comparing with isotype groups, the differences of nNOS, ChAT, and DβH expression were all signiifcant (P<0.05).Conclusion: ENSCs express neurotransmitters including NO and Ache, with NO of dominance. While CNS-NSCs express neurotrans-mitters such as NO, Ache and NE, especially Ache. There is almost no GABA expression in either ENSCs or CNS-NSCs.