中国医药科学
中國醫藥科學
중국의약과학
CHINA MEDICINE AND PHARMACY
2014年
22期
87-89
,共3页
氨酪酸氯化钠注射液%α-吡咯烷酮%含量测定%高效液相色谱法
氨酪痠氯化鈉註射液%α-吡咯烷酮%含量測定%高效液相色譜法
안락산록화납주사액%α-필각완동%함량측정%고효액상색보법
Aminobutyric acid sodium chloride injection%α-pyrrolidone%Content determination%HPLC
目的：建立高效液相色谱法检测氨酪酸氯化钠注射液中α-吡咯烷酮含量的方法标准。方法选用CAPCELL PAK C18柱为色谱柱；流动相采用pH2.1的磷酸二氢钾溶液-甲醇（90：10）；流速维持在1.0mL/min；紫外检测波长设定为210nm；柱温为30℃；进样量为10μL。结果α-吡咯烷酮浓度为0.4～5.0mg/L 时其线性关系良好，r=0.9998，平均回收率为100.27%（RSD=0.54%，n=6）。结论本研究建立的HPLC法对于氨酪酸氯化钠注射液中的α-吡咯烷酮测定效果良好且方便快捷。
目的：建立高效液相色譜法檢測氨酪痠氯化鈉註射液中α-吡咯烷酮含量的方法標準。方法選用CAPCELL PAK C18柱為色譜柱；流動相採用pH2.1的燐痠二氫鉀溶液-甲醇（90：10）；流速維持在1.0mL/min；紫外檢測波長設定為210nm；柱溫為30℃；進樣量為10μL。結果α-吡咯烷酮濃度為0.4～5.0mg/L 時其線性關繫良好，r=0.9998，平均迴收率為100.27%（RSD=0.54%，n=6）。結論本研究建立的HPLC法對于氨酪痠氯化鈉註射液中的α-吡咯烷酮測定效果良好且方便快捷。
목적：건립고효액상색보법검측안락산록화납주사액중α-필각완동함량적방법표준。방법선용CAPCELL PAK C18주위색보주；류동상채용pH2.1적린산이경갑용액-갑순（90：10）；류속유지재1.0mL/min；자외검측파장설정위210nm；주온위30℃；진양량위10μL。결과α-필각완동농도위0.4～5.0mg/L 시기선성관계량호，r=0.9998，평균회수솔위100.27%（RSD=0.54%，n=6）。결론본연구건립적HPLC법대우안락산록화납주사액중적α-필각완동측정효과량호차방편쾌첩。
ObjectiveTo establish an HPLC standard method to assay the α-pyrrolidone content in aminobutyric acid sodium chloride injection. Methods A CAPCELL PAK C18 column was adopted, and potassium dihydrogen phosphate solution were diluted with water to 1000 mL as mobile phase, which adjusted to pH 2.1 with phosphoric acid)-methanol (90:10,v/v).The flow rate was 1.0mL/min, the detection wavelength was 210 nm and column temperature was 30℃.ResultsThe linear correlation of aminobutyric acid was fine within 0.4-5.0mg/L(r=0.9998). The average recovery was 100.27% (RSD=0.54%,n=6). ConclusionThe HPLC method we established was good and convenient to determine the aminobutyric acid contents in amino butyric acid sodium chloride injection.
