CAJ | 학술논문

在不同温度下培养黑豆，提取不同萌发时期的酸性磷酸酯酶，利用福林-酚法测定其活力，研究温度对黑豆萌发时酸性磷酸酯酶活力的影响。结果表明，在15~35℃的温度范围内，黑豆在萌发时期，酸性磷酸酯酶的活力总体呈现出先上升、达到最大后又略微降低的现象。温度较高时，黑豆萌发较快，酸性磷酸酯酶达到最大酶活力所需时间较短；温度较低时，黑豆萌发过程中的最大酸性磷酸酯酶活力要低于较高温度下萌发的最大酶活力。
재불동온도하배양흑두，제취불동맹발시기적산성린산지매，이용복림-분법측정기활력，연구온도대흑두맹발시산성린산지매활력적영향。결과표명，재15~35℃적온도범위내，흑두재맹발시기，산성린산지매적활력총체정현출선상승、체도최대후우략미강저적현상。온도교고시，흑두맹발교쾌，산성린산지매체도최대매활력소수시간교단；온도교저시，흑두맹발과정중적최대산성린산지매활력요저우교고온도하맹발적최대매활력。
To study the effect of temperature on acid phosphatase activity during the period of black soy?bean germination,black soybeans were cultivated under different temperatures,and acid phosphatase from different periods of germination was extracted,then the acid phosphatase enzyme activity was determined us?ing Folin-phenol method.Research shows that,in the temperature range of 15~35℃,acid phosphatase activi?ty during germination first increases,and then slightly decreases after reaching its maximum.Black soybeans germinate rapidly under higher temperature,and acid phosphatase activity reaches maximum in shorter time. Besides,the maximum activity of acid phosphatase from black soybeans cultivated under lower temperature is less than that of higher temperature.