CAJ | 학술논문

目的：研究肝性脑病大鼠血脑屏障基质金属蛋白酶－9（MMP－9）和紧密连接蛋白－1（ZO－1）的表达对脑水肿的影响。方法将90只大鼠随机分为2组（对照组与肝性脑病组），应用 D－氨基半乳糖（400mg／kg）和内毒素（100μg／kg）联合腹腔内注射建立肝性脑病大鼠模型，观察大鼠肝性脑病症状。12h 后检测血氨浓度、利用伊文思蓝检测血脑屏障通透性、通过脑组织含水量测定判断脑水肿程度、Real－time Q－PCR 法检测血脑屏障 MMP－9 mRNA 和 ZO－1 mRNA 含量。结果造模2h 后肝性脑病组大鼠逐渐出现肝性脑病症状，造模后12h 大鼠血氨浓度较对照组明显升高[（279．53±9．78）μmol／L vs （33．85±1．50）μmol／L， P ＜0．01]，EB 含量较对照组明显升高[（6．71±0．50）μg／g vs （3．96±0．48）μg／g， P ＜0．01]，脑组织含水量较对照组明显升高[（82．14±0．30）％ vs （76．07±0．39）％， P ＜0．01]，脑组织 MMP－9 mRNA 含量较对照组明显升高（1．4255±0．1131 vs 0．8298±0．0537， P ＜0．01），而脑组织 ZO－1 mRNA 含量较对照组明显降低（1．0795±0．0534 vs 1．4576±0．0654， P＜0．01）。结论肝性脑病时出现血脑屏障通透性增强及脑水肿，可能与 MMP－9 mRNA 表达升高及 ZO－1 mRNA 表达下降有关。
목적：연구간성뇌병대서혈뇌병장기질금속단백매－9（MMP－9）화긴밀련접단백－1（ZO－1）적표체대뇌수종적영향。방법장90지대서수궤분위2조（대조조여간성뇌병조），응용 D－안기반유당（400mg／kg）화내독소（100μg／kg）연합복강내주사건립간성뇌병대서모형，관찰대서간성뇌병증상。12h 후검측혈안농도、이용이문사람검측혈뇌병장통투성、통과뇌조직함수량측정판단뇌수종정도、Real－time Q－PCR 법검측혈뇌병장 MMP－9 mRNA 화 ZO－1 mRNA 함량。결과조모2h 후간성뇌병조대서축점출현간성뇌병증상，조모후12h 대서혈안농도교대조조명현승고[（279．53±9．78）μmol／L vs （33．85±1．50）μmol／L， P ＜0．01]，EB 함량교대조조명현승고[（6．71±0．50）μg／g vs （3．96±0．48）μg／g， P ＜0．01]，뇌조직함수량교대조조명현승고[（82．14±0．30）％ vs （76．07±0．39）％， P ＜0．01]，뇌조직 MMP－9 mRNA 함량교대조조명현승고（1．4255±0．1131 vs 0．8298±0．0537， P ＜0．01），이뇌조직 ZO－1 mRNA 함량교대조조명현강저（1．0795±0．0534 vs 1．4576±0．0654， P＜0．01）。결론간성뇌병시출현혈뇌병장통투성증강급뇌수종，가능여 MMP－9 mRNA 표체승고급 ZO－1 mRNA 표체하강유관。
Objective To study the effect of MMP-9 and ZO-1 expressions on the brain edema in rats with hepatic encepha -lopathy.Methods Ninety rats were randomly divided into two groups .In one group, rats were intraperitoneal of D -galactosamine (400mg/kg) with lipopolysaccharide(100μg/kg) to induce hepatic encephalopathy , and then observe the symptom.12 hours later, ammonia, the evans blue(EB)content, the brain water content and the MMP -9 mRNA and the ZO-1 mRNA in the brain tissue was as-sessed by real-time Q-PCR.Results Two hours later the rats in hepatic encephalopathy groups begin to appear the symptom .12 hours later, the ammonia, the EB content, the brain water content and the MMP -9 mRNA content of the hepatic encephalopathy groups was increased significantly[(279.53 ±9.78)μmol /L vs (33.85 ±1.50)μmol/L, (6.71 ±0.50)μg/g vs (3.96 ±0.48)μg/g, (82.14 ±0.30)% vs (76.07 ±0.39)% and 1.4255 ±0.1131 vs 0.8298 ±0.0537, P <0.01],but the ZO-1 mRNA content was decreased (1.0795 ±0.0534 vs 1.4576 ±0.0654, P <0.01).Conclusions Blood brain barrier permeability enhancement and brain edema in hepatic encephalopathy rats, MMP-9 mRNA increase and ZO-1 mRNA decrease may play an important role .