CAJ | 학술논문

目的：探讨葛根素对过氧化氢诱导人视网膜色素上皮细胞（ ARPE－19 cells ）活力损伤和凋亡的影响。方法用200μmol／L过氧化氢（ H2 O2）诱导氧化应激，不同浓度葛根素作用于ARPE－19细胞24 h，应用WST－1法检测细胞活力，应用流式细胞仪检测细胞凋亡率。结果过氧化氢明显降低人视网膜色素上皮细胞活力，葛根素能剂量依赖性的减少这种降低；过氧化氢明显增加人视网膜色素上皮细胞的凋亡，葛根素能剂量依赖性的减少这种增加。结论葛根素能明显减少过氧化氢诱导的人视网膜色素上皮细胞活力的损伤和细胞凋亡。
목적：탐토갈근소대과양화경유도인시망막색소상피세포（ ARPE－19 cells ）활력손상화조망적영향。방법용200μmol／L과양화경（ H2 O2）유도양화응격，불동농도갈근소작용우ARPE－19세포24 h，응용WST－1법검측세포활력，응용류식세포의검측세포조망솔。결과과양화경명현강저인시망막색소상피세포활력，갈근소능제량의뢰성적감소저충강저；과양화경명현증가인시망막색소상피세포적조망，갈근소능제량의뢰성적감소저충증가。결론갈근소능명현감소과양화경유도적인시망막색소상피세포활력적손상화세포조망。
Objective To investigate the effect of puerarin on H 2 O2-induced viability loss and apoptosis of ARPE-19 cells.Methods ARPE-19 cells exposed to 200 μmol/L hydrogen per-oxide ( H2 O2 ) were incubated with various concentrations of puerarin for 24 h.Cell viability was detected by WST-1 assay .The cell apoptosis rate was measured by flow cytometry .Results H2 O2 reduced cell viability of ARPE-19 cells.Puerarin reduced H 2 O2-induced cell viability loss in a dose-dependent manner .H2 O2 increased cell apoptosis of ARPE-19 cells.Puerarin re-duced H2 O2-induced cell apoptosis in a dose-dependent manner .Conclusion Puerarin could clearly reduce H2 O2-induced viability loss and apoptosis of ARPE-19 cells.