CAJ | 학술논문

目的研究大明胶囊（Daming capsule，DM）对链脲佐菌素（streptozocin，STZ）诱导的1型糖尿病大鼠的血糖影响。方法将65只成年雄性Sprague－Dawley（SD）大鼠随机分为5组：DM低（50 mg?kg－1? d－1）、中（100 mg? kg－1? d－1）、高（200 mg? kg－1? d－1）剂量组、空白组及模型组。灌胃给予大鼠不同浓度的DM，空白组和模型组给予同等体积的生理盐水，每天两次。两周后，腹腔注射STZ 65 mg? kg －1建立1型糖尿病模型。 STZ注射后3天、7天检测大鼠空腹血糖，1个月后检测糖耐量。苏木素－伊红（ HE）染色和末端脱氧核糖核苷酸转移酶介导的缺口末端标记法（ TUNEL）观察各组胰岛形态学和凋亡。结果 DM显著降低STZ诱导糖尿病大鼠的空腹血糖值并改善糖尿病大鼠的糖耐量；在STZ大鼠，HE染色显示DM升高STZ大鼠胰岛的数量；TUNEL染色结果显示DM组的胰岛β细胞凋亡较STZ模型组显著减少。结论预防性给予DM能降低STZ诱导的1型糖尿病大鼠空腹血糖并明显改善糖耐量，减少胰岛β细胞凋亡。
목적연구대명효낭（Daming capsule，DM）대련뇨좌균소（streptozocin，STZ）유도적1형당뇨병대서적혈당영향。방법장65지성년웅성Sprague－Dawley（SD）대서수궤분위5조：DM저（50 mg?kg－1? d－1）、중（100 mg? kg－1? d－1）、고（200 mg? kg－1? d－1）제량조、공백조급모형조。관위급여대서불동농도적DM，공백조화모형조급여동등체적적생리염수，매천량차。량주후，복강주사STZ 65 mg? kg －1건립1형당뇨병모형。 STZ주사후3천、7천검측대서공복혈당，1개월후검측당내량。소목소－이홍（ HE）염색화말단탈양핵당핵감산전이매개도적결구말단표기법（ TUNEL）관찰각조이도형태학화조망。결과 DM현저강저STZ유도당뇨병대서적공복혈당치병개선당뇨병대서적당내량；재STZ대서，HE염색현시DM승고STZ대서이도적수량；TUNEL염색결과현시DM조적이도β세포조망교STZ모형조현저감소。결론예방성급여DM능강저STZ유도적1형당뇨병대서공복혈당병명현개선당내량，감소이도β세포조망。
Objective To study the protective effects of Daming capsule ( DM) in type 1 dia-betic rats.Methods Sixty-five male sprague-dawley (SD) rats were randomly divided into 5 groups (13 rats/group):low dose group (50 mg? kg -1? d -1 ), middle dose group (100 mg? kg -1? d-1 ) , high dose group of DM ( 200 mg? kg -1? d-1 ) , control group , and diabetes group .DM group received DM twice a day via gavage for two weeks;control group and diabe-tes group received equal volume of distilled water .Rats were administered with a single intra-peritoneal ( i.p.) injection of streptozocin ( STZ) 65 mg? kg -1 after two weeks of DM .The fasting blood glucose was measured on day 3 and 7 after STZ treatment and glucose tolerance test was performed on day 30 after STZ treatment.Hematoxylin and eosin stain (HE stain) and terminal-deoxynucleotidyl transferase mediated nick end labeling ( TUNEL ) assay were per-formed on the pancreas of the rats .Results Significant increase in the fasting blood glucose and abnormal glucose tolerance were found in the STZ-treated rats compared to controls ( P<0.05 ) .Rats treated with different doses of DM had significant decrease in the fast blood glu-cose ( P <0.05 ) and profound improvement in glucose tolerance in diabetic rats .HE stain showed that DM protected pancreatic islet against STZ damage , reflected by increase of number of pancreatic islets in diabetic rats ( P<0.05 ) .TUNEL results showed that DM attenuated ap-optosis of pancreatic β-cells compared with diabetes group .Conclusion DM decreases the fasting blood glucose level and improves glucose tolerance by at least protecting β-cells from ap-optosis in type 1 diabetic rats .