CAJ | 학술논문

目的在 mRNA 水平探讨内质网应激对3T3-L1脂肪细胞成纤维细胞生长因子21表达的影响。 　　方法利用经典鸡尾酒法诱导3T3-L1前脂肪细胞分化，再用毒胡萝卜内酯分别按照剂量梯度（0、6.25、12.5、25、50和100 nmol/L）和时间梯度（0、1、3、6和12 h）进行处理，实时 PCR 检测内质网应激相关蛋白 C/EBP 同源蛋白和葡萄糖调节蛋白78以及成纤维细胞生长因子21 mRNA的表达水平。 　　结果毒胡萝卜内酯能诱导分化成熟的3T3-L1脂肪细胞发生内质网应激，促进 C/EBP 同源蛋白、葡萄糖调节蛋白78和成纤维细胞生长因子21 mRNA 水平显著升高，且与毒胡萝卜内酯的剂量和处理时间呈正相关。当用100 nmol/L毒胡萝卜内酯处理6 h 后，成纤维细胞生长因子21 mRNA水平显著增高，为对照组的114.55倍（P<0.05）。蛋白激酶 C 抑制剂钙磷酸蛋白 C 能阻断由毒胡萝卜内酯诱导的内质网应激，并伴随成纤维细胞生长因子21 mRNA 水平的下降。 　　结论内质网应激能上调3T3-L1脂肪细胞成纤维细胞生长因子21 mRNA 表达，且可被蛋白激酶 C 抑制剂钙磷酸蛋白 C 阻断。
목적재 mRNA 수평탐토내질망응격대3T3-L1지방세포성섬유세포생장인자21표체적영향。 　　방법이용경전계미주법유도3T3-L1전지방세포분화，재용독호라복내지분별안조제량제도（0、6.25、12.5、25、50화100 nmol/L）화시간제도（0、1、3、6화12 h）진행처리，실시 PCR 검측내질망응격상관단백 C/EBP 동원단백화포도당조절단백78이급성섬유세포생장인자21 mRNA적표체수평。 　　결과독호라복내지능유도분화성숙적3T3-L1지방세포발생내질망응격，촉진 C/EBP 동원단백、포도당조절단백78화성섬유세포생장인자21 mRNA 수평현저승고，차여독호라복내지적제량화처리시간정정상관。당용100 nmol/L독호라복내지처리6 h 후，성섬유세포생장인자21 mRNA수평현저증고，위대조조적114.55배（P<0.05）。단백격매 C 억제제개린산단백 C 능조단유독호라복내지유도적내질망응격，병반수성섬유세포생장인자21 mRNA 수평적하강。 　　결론내질망응격능상조3T3-L1지방세포성섬유세포생장인자21 mRNA 표체，차가피단백격매 C 억제제개린산단백 C 조단。
Objective To study the effect of endoplasmic reticulum stress on FGF21 expression in the differentiated 3T3-L1 cells. Methods 3T3-L1 pre-adipocytes were cultured in vitro and differentiated into adipocytes, and then treated with different concentrations of thapsigargin (TG) (0, 6.25, 12.5, 25, 50 and 100 nmol/L) for 6 h or 100 nmol/L TG for different time (0, 1, 3, 6 and 12 h). The mRNA levels of CHOP, GRP78 and FGF21 were detected using real time PCR. Results TG-induced endoplasmic reticulum stress increased mRNA expression of CHOP, GRP78 and FGF21 in a dose-and time-dependent manner. FGF21 mRNA expression in 3T3-L1 adipocytes treated by 100 nmol/L TG for 6 h was significantly higher than that of control group (P < 0.05). PKC inhibitor calphostin C markedly inhibited TG-induceed endoplasmic reticulum stress, and reduced FGF21 mRNA expression. Conclusion FGF21 mRNA expression can be up-regulated by endoplasmic reticulum stress, which can be inhibited by PKC inhibitor calphostin C.