CAJ | 학술논문

目的：检测结扎大鼠双侧颈内动脉及一侧椎动脉（3?VO）是否可以在保留颈外动脉的同时，造成大鼠慢性脑缺血。方法分别制造假手术对照组、双侧颈总动脉结扎组（2?VO）及3?VO组动物模型；于造模4周后行脑血流量测定；于第8周行Morris水迷宫实验测定大鼠的学习记忆能力；行为学实验结束后处死大鼠，观察大鼠海马CA1区细胞的形态学变化。结果脑血流测定结果显示与假手术[（47±8．797） ml·min－1·100 g－1]相比，2?VO[（24．30±8．999）ml·min－1·100 g－1]、3?VO[（9．870±2．208）ml·min－1·100 g－1]组脑血流量值均降低，差异有统计学意义（P＜0．01）。 Morris水迷宫实验显示，2?VO组[（14．78±7．84） s]、3?VO[（14．86±7．96）s]组第5天潜伏期与假手术组[（8．33±4．88）s]相比，时间较长，差异有统计学意义（P＜0．01），但2?VO组与3?VO组相比，其潜伏期无明显差异；与假手术组[（37．20±9．21）s，（10．01±2．91）次]相比，2?VO组[（20．13±5．80）s，（6．60±3．19）次]、3?VO组[（20．05±5．76）s，（6．55±2．59）次]目标象限停留时间及穿越平台次数均显著减少，差异有统计学意义（P＜0．01）。2?VO、3?VO模型组海马CA1区有明显病理形态学改变。结论结扎双侧颈内动脉及一侧椎动脉可造成大鼠慢性脑缺血，并造成与2?VO相似的行为学表现。
목적：검측결찰대서쌍측경내동맥급일측추동맥（3?VO）시부가이재보류경외동맥적동시，조성대서만성뇌결혈。방법분별제조가수술대조조、쌍측경총동맥결찰조（2?VO）급3?VO조동물모형；우조모4주후행뇌혈류량측정；우제8주행Morris수미궁실험측정대서적학습기억능력；행위학실험결속후처사대서，관찰대서해마CA1구세포적형태학변화。결과뇌혈류측정결과현시여가수술[（47±8．797） ml·min－1·100 g－1]상비，2?VO[（24．30±8．999）ml·min－1·100 g－1]、3?VO[（9．870±2．208）ml·min－1·100 g－1]조뇌혈류량치균강저，차이유통계학의의（P＜0．01）。 Morris수미궁실험현시，2?VO조[（14．78±7．84） s]、3?VO[（14．86±7．96）s]조제5천잠복기여가수술조[（8．33±4．88）s]상비，시간교장，차이유통계학의의（P＜0．01），단2?VO조여3?VO조상비，기잠복기무명현차이；여가수술조[（37．20±9．21）s，（10．01±2．91）차]상비，2?VO조[（20．13±5．80）s，（6．60±3．19）차]、3?VO조[（20．05±5．76）s，（6．55±2．59）차]목표상한정류시간급천월평태차수균현저감소，차이유통계학의의（P＜0．01）。2?VO、3?VO모형조해마CA1구유명현병리형태학개변。결론결찰쌍측경내동맥급일측추동맥가조성대서만성뇌결혈，병조성여2?VO상사적행위학표현。
Objective To estimate whether the ligation of bilateral internal carotid artery in combination with one vertebral artery can lead to chronic cerebral hypoperfusion. Methods The sham operation, 2?VO and 3?VO rat models were subjected to the matching operation. Four weeks after operation,the cortical blood flow was determined. The learning and memory abilities were measured with Morris water maze test eight weeks later,then the rats were sacrificed to observe the morphological change of hippocampal CA1 region. Results Compared with the sham operation group((47±8.797)ml·min-1·100 g-1),the cerebral blood flow of 2?VO((24.30±8.999)ml ·min-1·100 g-1) and 3?VO((9.870±2.208)ml·min-1·100 g-1) were significantly decreased (P<0.01). Compared with the sham operation group((8.33±4.88)s),escape latencies of Morris water maze of 2?VO group ((14.78±7.84)s) and 3?VO group((14.86±7.96)s) in the fifth days also presented significantly increased (P<0.01),but rare difference between the two groups. Compared with the sham operation group[ (37.20±9.21) s, (10.01.±2.91)times],the target quadrant swimming time and crossing times of 2?VO group((20.13±5.80)s, (6.60±3.19)times) and 3?VO group((20.05±5.76)s,(6.55±2.59)times) in the fifth days also presented signifi?cantly decreased (P<0.01). There were distinct pathomorphology changes in hippocampal CA1 region of the two groups. Conclusion The ligation of bilateral internal carotid artery in combination with one vertebral artery can lead to chronic cerebral hypoperfusion,and can make the similar ethology representation with the 2?VO models.