应用化工
應用化工
응용화공
APPLIED CHEMICAL INDUSTRY
2014年
11期
2123-2126
,共4页
尿酸%化学发光%无毒%稳定%简便
尿痠%化學髮光%無毒%穩定%簡便
뇨산%화학발광%무독%은정%간편
uric acid%chemiluminescence%non-toxic%stable%simple
检测人尿液中尿酸含量的化学发光新方法，试剂稳定、无毒，不使用鲁米诺。在碱性条件下，尿酸选择性地猝灭抗坏血酸与荧光素-十六烷基三甲基溴化铵-铜离子混合物体系的化学发光，尿样直接稀释后化学发光检测尿酸含量。尿酸浓度在0.5~10 mg/L的范围内与化学发光信号具有良好的线性关系，线性回归方程为ΔI =306.6 c+10.904（n=5，R2=0.990），最低检出限为0.06 mg/L（3σ），定量检出限为0.2 mg/L（10σ），对浓度1 mg/L的尿酸平行测定n=11，RSD为1.2%。将本法用于人体尿液中尿酸含量的测定，与临床测定结果进行了对比，并进行了回收率实验，说明本方法具有较好的准确性和选择性；结合流动注射技术操作简便，对临床检验有参考价值和转化应用潜质。
檢測人尿液中尿痠含量的化學髮光新方法，試劑穩定、無毒，不使用魯米諾。在堿性條件下，尿痠選擇性地猝滅抗壞血痠與熒光素-十六烷基三甲基溴化銨-銅離子混閤物體繫的化學髮光，尿樣直接稀釋後化學髮光檢測尿痠含量。尿痠濃度在0.5~10 mg/L的範圍內與化學髮光信號具有良好的線性關繫，線性迴歸方程為ΔI =306.6 c+10.904（n=5，R2=0.990），最低檢齣限為0.06 mg/L（3σ），定量檢齣限為0.2 mg/L（10σ），對濃度1 mg/L的尿痠平行測定n=11，RSD為1.2%。將本法用于人體尿液中尿痠含量的測定，與臨床測定結果進行瞭對比，併進行瞭迴收率實驗，說明本方法具有較好的準確性和選擇性；結閤流動註射技術操作簡便，對臨床檢驗有參攷價值和轉化應用潛質。
검측인뇨액중뇨산함량적화학발광신방법，시제은정、무독，불사용로미낙。재감성조건하，뇨산선택성지졸멸항배혈산여형광소-십륙완기삼갑기추화안-동리자혼합물체계적화학발광，뇨양직접희석후화학발광검측뇨산함량。뇨산농도재0.5~10 mg/L적범위내여화학발광신호구유량호적선성관계，선성회귀방정위ΔI =306.6 c+10.904（n=5，R2=0.990），최저검출한위0.06 mg/L（3σ），정량검출한위0.2 mg/L（10σ），대농도1 mg/L적뇨산평행측정n=11，RSD위1.2%。장본법용우인체뇨액중뇨산함량적측정，여림상측정결과진행료대비，병진행료회수솔실험，설명본방법구유교호적준학성화선택성；결합류동주사기술조작간편，대림상검험유삼고개치화전화응용잠질。
Development of new luminol free chemiluminescence method for determination of uric acid in human urine with the use of non-toxic and stable chemiluminescent reagents. In alkaline solution,chemi-luminescence produced by ascorbic acid reacting with fluorescein-CTMAB-copper ion can be selectively quenched by the presence of uric acid,diluted urine samples were analyzed by proposed chemilumines-cence method. The uric acid concentration in the range of 0. 5 to 10 mg/L and chemiluminescence signal has a good liner relationship,obeyed the equation △I=306. 6 c+10. 904(n=5,R2 =0. 990)with the lowest detection limit of 0 . 06 mg/L( 3σ)and quantification limit of 0 . 2 mg/L( 10σ). The relative standard deviation(RSD)was 1. 2%(n=11)when determining 1. 0 mg/L uric acid standard. By meas-uring the real urine samples in contrast to the clinical results,as well as by the recovery test,the proposed method presented acceptable selectivity,accuracy and sensitivity for the determination of uric acid. In combination with flow injection,the new method achieved simple and robust analytical operation. As such, this method implies its potential as an alternative to be developed for clinical application.
