中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2014年
11期
961-963
,共3页
姚刚%郝婷婷%黄倩%于挺敏
姚剛%郝婷婷%黃倩%于挺敏
요강%학정정%황천%우정민
利扎曲普坦%偏头痛%中脑导水管周围灰质%缩胆囊肽
利扎麯普坦%偏頭痛%中腦導水管週圍灰質%縮膽囊肽
리찰곡보탄%편두통%중뇌도수관주위회질%축담낭태
Rizatriptan%Migraine%Periaqueductal gray%Cholecystokinin
目的：观察利扎曲普坦对偏头痛大鼠中脑导水管周围灰质（ Periaqueductal gray，PAG）缩胆囊肽（ cholecystokinin，CCK）表达的影响，探讨曲普坦类药物对偏头痛发作时内源性痛觉调制系统的干预作用。方法健康Wistar大鼠随机分4组：A组：对照组，B组：偏头痛组，C组：利扎曲普坦对照组，D组：利扎曲普坦治疗组。利扎曲普坦给药组（ C、D组）大鼠给予利扎曲普坦1 mg／（ kg·d）灌胃。给药7 d后，B、D组大鼠制备硝酸甘油型偏头痛动物模型，造模2 h留取中脑标本。实时定量PCR及免疫组化法检测CCK的表达情况。结果 A、B、C、D组大鼠中脑每250 ng总RNA CCK mRNA拷贝数（×106）分别为：1．25±0．41、1．71±0．93、0．17±0．12、0．22±0．07。 A、B、C、D组大鼠PAG区CCK?8免疫反应阳性细胞个数分别为：37．17±12．62、40．17±11．09、27．33±7．71、20．67±7．66。 C组大鼠中脑CCK mRNA拷贝数明显低于A组（P＜0．05），D组大鼠中脑CCK mRNA拷贝数明显低于B组（P＜0．05）；D组大鼠中脑PAG区CCK?8免疫反应阳性细胞数少于B组（P＜0．05）。结论利扎曲普坦能够下调偏头痛大鼠中脑CCK基因表达，减弱CCK?8对内源性阿片肽镇痛效应的抑制作用，从而提高阿片肽镇痛效应，增强内源性痛觉调制系统的镇痛作用。
目的：觀察利扎麯普坦對偏頭痛大鼠中腦導水管週圍灰質（ Periaqueductal gray，PAG）縮膽囊肽（ cholecystokinin，CCK）錶達的影響，探討麯普坦類藥物對偏頭痛髮作時內源性痛覺調製繫統的榦預作用。方法健康Wistar大鼠隨機分4組：A組：對照組，B組：偏頭痛組，C組：利扎麯普坦對照組，D組：利扎麯普坦治療組。利扎麯普坦給藥組（ C、D組）大鼠給予利扎麯普坦1 mg／（ kg·d）灌胃。給藥7 d後，B、D組大鼠製備硝痠甘油型偏頭痛動物模型，造模2 h留取中腦標本。實時定量PCR及免疫組化法檢測CCK的錶達情況。結果 A、B、C、D組大鼠中腦每250 ng總RNA CCK mRNA拷貝數（×106）分彆為：1．25±0．41、1．71±0．93、0．17±0．12、0．22±0．07。 A、B、C、D組大鼠PAG區CCK?8免疫反應暘性細胞箇數分彆為：37．17±12．62、40．17±11．09、27．33±7．71、20．67±7．66。 C組大鼠中腦CCK mRNA拷貝數明顯低于A組（P＜0．05），D組大鼠中腦CCK mRNA拷貝數明顯低于B組（P＜0．05）；D組大鼠中腦PAG區CCK?8免疫反應暘性細胞數少于B組（P＜0．05）。結論利扎麯普坦能夠下調偏頭痛大鼠中腦CCK基因錶達，減弱CCK?8對內源性阿片肽鎮痛效應的抑製作用，從而提高阿片肽鎮痛效應，增彊內源性痛覺調製繫統的鎮痛作用。
목적：관찰리찰곡보탄대편두통대서중뇌도수관주위회질（ Periaqueductal gray，PAG）축담낭태（ cholecystokinin，CCK）표체적영향，탐토곡보탄류약물대편두통발작시내원성통각조제계통적간예작용。방법건강Wistar대서수궤분4조：A조：대조조，B조：편두통조，C조：리찰곡보탄대조조，D조：리찰곡보탄치료조。리찰곡보탄급약조（ C、D조）대서급여리찰곡보탄1 mg／（ kg·d）관위。급약7 d후，B、D조대서제비초산감유형편두통동물모형，조모2 h류취중뇌표본。실시정량PCR급면역조화법검측CCK적표체정황。결과 A、B、C、D조대서중뇌매250 ng총RNA CCK mRNA고패수（×106）분별위：1．25±0．41、1．71±0．93、0．17±0．12、0．22±0．07。 A、B、C、D조대서PAG구CCK?8면역반응양성세포개수분별위：37．17±12．62、40．17±11．09、27．33±7．71、20．67±7．66。 C조대서중뇌CCK mRNA고패수명현저우A조（P＜0．05），D조대서중뇌CCK mRNA고패수명현저우B조（P＜0．05）；D조대서중뇌PAG구CCK?8면역반응양성세포수소우B조（P＜0．05）。결론리찰곡보탄능구하조편두통대서중뇌CCK기인표체，감약CCK?8대내원성아편태진통효응적억제작용，종이제고아편태진통효응，증강내원성통각조제계통적진통작용。
Objective To assess the influence of Rizatriptan on the cholecystokinin( CCK) expression in periaqueductal gray( PAG) of migraine model rat to investigate the possible mechanism by which Triptans treat mi?graine. Methods A total of 24 rats were randomly divided into four groups:normal control groups(A),migraine model groups(B),Rizatriptan control groups(C) and Rizatriptan treatment groups(D).C and D groups were intra?gastrically perfused with Rizatriptan,1 mg/kg per day. After 7 days,nitroglycerin was subcutaneously injected into the buttocks of the B and D group to induce migraine. Two hours after nitroglycerin injection,the trigeminal ganglia were isolated.CGRP expression in periaqueductal gray were determined using SYBR Green I real?time quantitative PCR and Immunohistochemistry. Results CCK mRNA levels ( target gene mRNA copies per 250 ng total RNA,× 106) in the rat midbrain of A,B,C,D groups were 1.25±0.41,1.71±0.93,0.17±0.12,0.22±0.07 respectively. CCK?8?immunoreactive positive cells in the rat PAG of each group were 37.17±12.62,40.17±11.09,27.33±7.71, 20.67±7.66 respectively. CCK mRNA expression in group C was significantly lower than that of group A(P<0.05) while the CCK mRNA expression in group D was lower than that of group B(P<0.05).The CCK?8?positive cells of the rat PAG in group D were lower than that in group B(P<0.05) . Conclusion Rizatriptan can down regulate the expression of CCK?8 in the PAG of the migraine rats and weaken the CCK?8 induced inhibition of the analgesic effects of opioid peptides.
