山东化工
山東化工
산동화공
SHANDONG CHEMICAL INDUSTRY
2014年
11期
90-91,94
,共3页
邹亚楠%刘琦%王利娟%刘海燕%赵立春
鄒亞楠%劉琦%王利娟%劉海燕%趙立春
추아남%류기%왕리연%류해연%조립춘
红参%人参皂苷Rg1%高效液相色谱
紅參%人參皂苷Rg1%高效液相色譜
홍삼%인삼조감Rg1%고효액상색보
red ginseng%ginsenoside Rg1%high performance liquid chromatography
建立红参药材中人参皂苷Rg1的含量测定方法。利用高效液相色谱（ HPLC）法测定红参中人参皂苷Rg1的含量，采用Uliti-mate XB-C18（2）柱（250 mm ×4.6 mm，5μm）；流动相：乙腈-水（23：77）；柱温：室温；流速：0.7 mL·min-1；检测波长：203 nm；进样量为10μL。人参皂苷Rg1的线性回归方程为A=438355.8C-1328.9（r =0.9999）。人参皂苷Rg1在0.1~5μg范围内呈良好的线性关系。人参皂苷Rg1的平均回收率为103.6％，RSD为1.581％。采用此法测定红参中人参皂苷Rg1的含量，准确可靠，可用于该药材的质量控制。
建立紅參藥材中人參皂苷Rg1的含量測定方法。利用高效液相色譜（ HPLC）法測定紅參中人參皂苷Rg1的含量，採用Uliti-mate XB-C18（2）柱（250 mm ×4.6 mm，5μm）；流動相：乙腈-水（23：77）；柱溫：室溫；流速：0.7 mL·min-1；檢測波長：203 nm；進樣量為10μL。人參皂苷Rg1的線性迴歸方程為A=438355.8C-1328.9（r =0.9999）。人參皂苷Rg1在0.1~5μg範圍內呈良好的線性關繫。人參皂苷Rg1的平均迴收率為103.6％，RSD為1.581％。採用此法測定紅參中人參皂苷Rg1的含量，準確可靠，可用于該藥材的質量控製。
건립홍삼약재중인삼조감Rg1적함량측정방법。이용고효액상색보（ HPLC）법측정홍삼중인삼조감Rg1적함량，채용Uliti-mate XB-C18（2）주（250 mm ×4.6 mm，5μm）；류동상：을정-수（23：77）；주온：실온；류속：0.7 mL·min-1；검측파장：203 nm；진양량위10μL。인삼조감Rg1적선성회귀방정위A=438355.8C-1328.9（r =0.9999）。인삼조감Rg1재0.1~5μg범위내정량호적선성관계。인삼조감Rg1적평균회수솔위103.6％，RSD위1.581％。채용차법측정홍삼중인삼조감Rg1적함량，준학가고，가용우해약재적질량공제。
To establish a method to determine the content of ginsenoside Rg1 in red ginseng. HPLC was used for the determination of ginsenoside Rg1 in red ginseng. This method was established on a Ulitimate XB-C18(2)column with the mobile phase consisting of acetonitrile-water(23:77)and the flow rate of 0. 7 mL·min-1 at the room temperature,the detection wavelength was 203 nm. The injection volume was 10 μL. The regression equation of ginsenoside Rg1 was A=438355. 8C-1328. 9(r = 0. 9999). The ginsenoside Rg1 had a good linear relationship in the range of 0. 1~5 μg. And the average recoveries of ginsenoside Rg1 were 103. 6%,RSD was 1. 581%. The method for the determination of ginsenoside Rg1 content in red ginseng is accurate and reliable,and it can be used for the quality control of the medicines.
