山东化工
山東化工
산동화공
SHANDONG CHEMICAL INDUSTRY
2014年
11期
42-44
,共3页
浒苔%孢子%抗生素%基因工程%选择标记
滸苔%孢子%抗生素%基因工程%選擇標記
호태%포자%항생소%기인공정%선택표기
Ulva linza%spores%antibiotics%genetic engineering%screening marker
选择标记的确定是基因工程研究的一个重要环节。主要研究了缘管浒苔游孢子对青霉素、氯霉素等9种抗生素的敏感性，并与不同发育时期的藻体进行了比较。结果表明：缘管浒苔游孢子对氯霉素、新霉素、红霉素、G-418敏感性较高，在10μg/mL时均有明显的致死效应；不同发育时期浒苔的抗生素敏感性不同，成熟度越高，敏感性越低；对青霉素、卡那霉素、四环素、潮霉素、链霉素不敏感。本实验结果提示氯霉素、红霉素、G-418、新霉素可作为缘管浒苔游孢子转基因研究理想的选择试剂。
選擇標記的確定是基因工程研究的一箇重要環節。主要研究瞭緣管滸苔遊孢子對青黴素、氯黴素等9種抗生素的敏感性，併與不同髮育時期的藻體進行瞭比較。結果錶明：緣管滸苔遊孢子對氯黴素、新黴素、紅黴素、G-418敏感性較高，在10μg/mL時均有明顯的緻死效應；不同髮育時期滸苔的抗生素敏感性不同，成熟度越高，敏感性越低；對青黴素、卡那黴素、四環素、潮黴素、鏈黴素不敏感。本實驗結果提示氯黴素、紅黴素、G-418、新黴素可作為緣管滸苔遊孢子轉基因研究理想的選擇試劑。
선택표기적학정시기인공정연구적일개중요배절。주요연구료연관호태유포자대청매소、록매소등9충항생소적민감성，병여불동발육시기적조체진행료비교。결과표명：연관호태유포자대록매소、신매소、홍매소、G-418민감성교고，재10μg/mL시균유명현적치사효응；불동발육시기호태적항생소민감성불동，성숙도월고，민감성월저；대청매소、잡나매소、사배소、조매소、련매소불민감。본실험결과제시록매소、홍매소、G-418、신매소가작위연관호태유포자전기인연구이상적선택시제。
To determine the proper screening marker is a key step for genetic engineering. In this study the sensitivity of Ulva linza spores to nine kinds of antibiotics such as penicillin,chloramphenicol and so on was investigated,and it was also compared with Ulva linza in different development period. The results showed that Ulva linza spores were more sensitive to chloramphenicol,erythromycin,neomycin and G -418,all exhibiting obvious killing effects under the concentration of 10μg/mL,whereas mature alga was less sensitive;they were not sensitive to penicillin, kanamycin, homomycin, streptomycin and tetracycline. This indicated that chloramphenicol,erythromycin,neomycin and G-418 could be used as screening agents for gene transferring of Ulva linza spores.
