CAJ | 학술논문

目的：探索使陈旧性乳腺癌石蜡标本ER、PR、Ki-67在免疫组化检测中获得高表达的抗原修复时间。方法采用Envision二步法，将120例存放>2年的乳腺癌石蜡组织切片，分别在EDTA（pH9.0）修复液中高压修复5、10、15、20min和水浴修复30min，比较抗原表达差异。结果 ER、PR在修复时间>15min时阳性率明显提高，Ki-67在高压修复15、20min组阳性率较水浴30min、高压修复5、10min组明显提高。ER、PR、Ki-67在高压修复20min时阳性信号强，阳性率最高，与修复5、10min组差异有显著性（P<0.05）。结论高压修复20min可显著提高陈旧性乳腺癌石蜡组织ER、PR、Ki-67抗原的检出率。
목적：탐색사진구성유선암석사표본ER、PR、Ki-67재면역조화검측중획득고표체적항원수복시간。방법채용Envision이보법，장120례존방>2년적유선암석사조직절편，분별재EDTA（pH9.0）수복액중고압수복5、10、15、20min화수욕수복30min，비교항원표체차이。결과 ER、PR재수복시간>15min시양성솔명현제고，Ki-67재고압수복15、20min조양성솔교수욕30min、고압수복5、10min조명현제고。ER、PR、Ki-67재고압수복20min시양성신호강，양성솔최고，여수복5、10min조차이유현저성（P<0.05）。결론고압수복20min가현저제고진구성유선암석사조직ER、PR、Ki-67항원적검출솔。
Objective To explore the time of antigen retrieval with high expression for immunohistochemical staining of ER、PR、Ki-67 in old breast cancer paraffin. Methods Immunohistochemical staining of 120 old breast cancer paraffin cases stored for over 2 years was processed by Envision method and each tissue was retrieved in EDTA(pH9.0)antigen retrieval buffer under high pressure for 5, 10, 15 and 20 minutes respectively and water-bathed for 30 minutes;the antigen expressions were compared. Results The positive rates of ER and PR were obviously increased when the retrieval time was over 15 minutes;Ki-67 was of a higher positive rate when re-trieved under high-pressure for 15 and 20 minutes than when water-bathed for 30minutes and when retrieved under high pressure for 5 and 10 minutes;ER, Pr and Ki-67 showed strong positive signals and the highest positive rates when retrieved under high pressure for 20 minutes, significantly different from the positive rates when retrieved for 5 and 10 minutes(P<0.05). Conclusions Retrieval under high pressure for 20 minutes can obviously raise the detection rate of ER, PR and Ki-67 in old breast cancer paraffin.