CAJ | 학술논문

目的：分析比较不同年龄人群的血浆 N-糖基的结构谱型。方法采用整群抽样，以2009年4至7月在首都医科大学宣武医院进行健康体检的212名居民为研究对象，基于高效液相色谱法进行血浆糖基组检测。将年龄以中位数35为切点分组，不同年龄组的糖基含量比较采用两独立样本 t 检验或非参数检验。结果212名研究对象的平均年龄为(37.78±17.89)岁，性别比为0.88。无论男性女性，半乳糖基化水平，≥35岁组二半乳糖聚糖含量较高、双触角非半乳糖聚糖含量较低；在两分支糖基的唾液酸化水平，<35岁组二唾液酸化双触角聚糖含量较高；在唾液酸化水平，≥35岁组单唾液酸含量高、三唾液酸较低；岩藻糖基化位置，≥35岁组核心岩藻糖化聚糖含量高；在分支水平，≥35岁组双触角聚糖含量较高，三触角聚糖和四触角聚糖含量较低(P≤0.03)。结论血浆 N-糖基结构可能与年龄有关。
목적：분석비교불동년령인군적혈장 N-당기적결구보형。방법채용정군추양，이2009년4지7월재수도의과대학선무의원진행건강체검적212명거민위연구대상，기우고효액상색보법진행혈장당기조검측。장년령이중위수35위절점분조，불동년령조적당기함량비교채용량독립양본 t 검험혹비삼수검험。결과212명연구대상적평균년령위(37.78±17.89)세，성별비위0.88。무론남성녀성，반유당기화수평，≥35세조이반유당취당함량교고、쌍촉각비반유당취당함량교저；재량분지당기적타액산화수평，<35세조이타액산화쌍촉각취당함량교고；재타액산화수평，≥35세조단타액산함량고、삼타액산교저；암조당기화위치，≥35세조핵심암조당화취당함량고；재분지수평，≥35세조쌍촉각취당함량교고，삼촉각취당화사촉각취당함량교저(P≤0.03)。결론혈장 N-당기결구가능여년령유관。
Objective To explore difference of plasma N-glycosylation structure between age groups. Methods Based on cluster sampling, 212 residents of a certain community were selected and the serum N-glycans profiles were obtained by high performance liquid chromatography( HPLC). Two independent samples t test or nonparametric tests were used to compare the N-glycosylation structure between age groups divided by 35. Results The average age of the 212 subjects was(37. 78±17. 89) years, and the sex ratio was 0. 88. More digalactosylated glycans, monosialylated glycans, core-fucosylated glycans and biantennary glycans were detected in 35 years or more, whereas more biantennary non-galactosylated glycans, disialylated biantennary glycans, trisialylated glycans and triantennary glycans were seen in younger than 35 years(P≤0. 03). Conclusion The N-glycome profile in serum is age dependent. This should be taken into consideration in the development of serum glycome markers.