首都医科大学学报
首都醫科大學學報
수도의과대학학보
JOURNAL OF CAPITAL UNIVERSITY OF MEDICAL SCIENCES
2014年
6期
735-740
,共6页
张如意%张丽%吴燕川%李林
張如意%張麗%吳燕川%李林
장여의%장려%오연천%리림
二苯乙烯苷%1-甲基-4-苯基-1,2,3,6-四氢吡啶%帕金森病%行为学%多巴胺
二苯乙烯苷%1-甲基-4-苯基-1,2,3,6-四氫吡啶%帕金森病%行為學%多巴胺
이분을희감%1-갑기-4-분기-1,2,3,6-사경필정%파금삼병%행위학%다파알
tetrahydroxystilbene glucoside%1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine%Parkinson' s disease%behavior%eo-pamine
目的：观察二苯乙烯苷(tetrahydroxystilbene glucoside,TSG)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine,MPTP)拟帕金森病(Parkinson's disease,PD)模型小鼠行为学及多巴胺能神经元的影响。方法 C57BL小鼠按数字表法随机分为对照组、模型组、TSG 小剂量组(TSG 60 mg/ kg)和 TSG 大剂量组(TSG 120 mg/ kg)。 MPTP 30 mg/ kg 腹腔注射5 d 建立拟 PD 小鼠模型。采用爬杆实验、转棒实验和自主活动实验测试小鼠行为学变化。免疫组织化学法检测各组小鼠黑质处酪氨酸羟化酶(tyrosine hydroxylase,TH)阳性细胞的表达情况。高效液相色谱电化学检测法测定小鼠纹状体内多巴胺及其代谢产物的含量。结果与对照组相比,MPTP 模型组小鼠爬杆时间显著延长,在转棒上的停留时间明显缩短,自主活动数减少,脑黑质处 TH 阳性细胞数量显著减少,纹状体内多巴胺及其代谢产物含量显著降低。与模型组小鼠相比,TSG 用药组小鼠运动协调性显著增强,脑黑质处 TH 阳性细胞数量增加,纹状体内多巴胺含量升高。结论 TSG 可改善 MPTP 模型小鼠的行为学,保护黑质多巴胺能神经元。 TSG 可能具有潜在的防治 PD 的作用。
目的：觀察二苯乙烯苷(tetrahydroxystilbene glucoside,TSG)對1-甲基-4-苯基-1,2,3,6-四氫吡啶(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine,MPTP)擬帕金森病(Parkinson's disease,PD)模型小鼠行為學及多巴胺能神經元的影響。方法 C57BL小鼠按數字錶法隨機分為對照組、模型組、TSG 小劑量組(TSG 60 mg/ kg)和 TSG 大劑量組(TSG 120 mg/ kg)。 MPTP 30 mg/ kg 腹腔註射5 d 建立擬 PD 小鼠模型。採用爬桿實驗、轉棒實驗和自主活動實驗測試小鼠行為學變化。免疫組織化學法檢測各組小鼠黑質處酪氨痠羥化酶(tyrosine hydroxylase,TH)暘性細胞的錶達情況。高效液相色譜電化學檢測法測定小鼠紋狀體內多巴胺及其代謝產物的含量。結果與對照組相比,MPTP 模型組小鼠爬桿時間顯著延長,在轉棒上的停留時間明顯縮短,自主活動數減少,腦黑質處 TH 暘性細胞數量顯著減少,紋狀體內多巴胺及其代謝產物含量顯著降低。與模型組小鼠相比,TSG 用藥組小鼠運動協調性顯著增彊,腦黑質處 TH 暘性細胞數量增加,紋狀體內多巴胺含量升高。結論 TSG 可改善 MPTP 模型小鼠的行為學,保護黑質多巴胺能神經元。 TSG 可能具有潛在的防治 PD 的作用。
목적：관찰이분을희감(tetrahydroxystilbene glucoside,TSG)대1-갑기-4-분기-1,2,3,6-사경필정(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine,MPTP)의파금삼병(Parkinson's disease,PD)모형소서행위학급다파알능신경원적영향。방법 C57BL소서안수자표법수궤분위대조조、모형조、TSG 소제량조(TSG 60 mg/ kg)화 TSG 대제량조(TSG 120 mg/ kg)。 MPTP 30 mg/ kg 복강주사5 d 건립의 PD 소서모형。채용파간실험、전봉실험화자주활동실험측시소서행위학변화。면역조직화학법검측각조소서흑질처락안산간화매(tyrosine hydroxylase,TH)양성세포적표체정황。고효액상색보전화학검측법측정소서문상체내다파알급기대사산물적함량。결과여대조조상비,MPTP 모형조소서파간시간현저연장,재전봉상적정류시간명현축단,자주활동수감소,뇌흑질처 TH 양성세포수량현저감소,문상체내다파알급기대사산물함량현저강저。여모형조소서상비,TSG 용약조소서운동협조성현저증강,뇌흑질처 TH 양성세포수량증가,문상체내다파알함량승고。결론 TSG 가개선 MPTP 모형소서적행위학,보호흑질다파알능신경원。 TSG 가능구유잠재적방치 PD 적작용。
Objective To observe effects of tetrahydroxystilbene glucoside ( TSG) on behavior and content of dopamine and its metabolites in striatum of 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine(MPTP) model mice. Methods Mice were randomly divided into normal control group, model group, and TSG low dose(60 mg/ kg) and high dose(120 mg/ kg) groups. The behavior changes of mice were observed by pole test, Rotarod test and spontaneous movement test. The tyrosine hydroxylase(TH) positive cells were detected by immunohistochemical method. The content of dopamine(DA) and its metabolic products in striatum were determined by HPLC-ECD. Results MPTP model mice showed behavior deficit. The counts of TH positive neurons in substantia nigra and the content of dopamine and its metabolites in striatum in model mice decreased significantly compared with control group. TSG ameliorated the behavior deficits, increased the number of TH positive neurons in the substantia nigra, and elevated the content of dopamine in striatum compared with model mice. Conclusion TSG protected dopaminergic neurons against MPTP-induced damage, and may become a candidate drug for prevention and treatment of Parkinson's disease.
