热带亚热带植物学报
熱帶亞熱帶植物學報
열대아열대식물학보
JOURNAL OF TROPICAL AND SUBTROPICAL BOTANY
2014年
6期
617-623
,共7页
诺丽%ISSR%形态特征%遗传多样性
諾麗%ISSR%形態特徵%遺傳多樣性
낙려%ISSR%형태특정%유전다양성
Morinda citrifolia%ISSR%Morphological characteristics%Genetic diversity
为探讨我国诺丽(Morinda citrifolia)种质资源的亲缘关系,采用ISSR技术对诺丽种质资源的遗传关系进行分析。结果表明,10条ISSR引物对13份诺丽种质资源共扩增出183条带,其中多态性条带有159条,占86.9%。13份诺丽种质的遗传相似系数为0.464~0.784。聚类分析将13份诺丽种质资源聚为两类,其中诺丽小黑种单独聚为一类,与其他12份诺丽种质资源的亲缘关系较远。虽然按照外部形态特征不能将所有诺丽种质完全区分,但具有相同特征的多数种质还是聚在同一类或亚类中。
為探討我國諾麗(Morinda citrifolia)種質資源的親緣關繫,採用ISSR技術對諾麗種質資源的遺傳關繫進行分析。結果錶明,10條ISSR引物對13份諾麗種質資源共擴增齣183條帶,其中多態性條帶有159條,佔86.9%。13份諾麗種質的遺傳相似繫數為0.464~0.784。聚類分析將13份諾麗種質資源聚為兩類,其中諾麗小黑種單獨聚為一類,與其他12份諾麗種質資源的親緣關繫較遠。雖然按照外部形態特徵不能將所有諾麗種質完全區分,但具有相同特徵的多數種質還是聚在同一類或亞類中。
위탐토아국낙려(Morinda citrifolia)충질자원적친연관계,채용ISSR기술대낙려충질자원적유전관계진행분석。결과표명,10조ISSR인물대13빈낙려충질자원공확증출183조대,기중다태성조대유159조,점86.9%。13빈낙려충질적유전상사계수위0.464~0.784。취류분석장13빈낙려충질자원취위량류,기중낙려소흑충단독취위일류,여기타12빈낙려충질자원적친연관계교원。수연안조외부형태특정불능장소유낙려충질완전구분,단구유상동특정적다수충질환시취재동일류혹아류중。
In order to understand the genetic relationship ofMorinda citrifolia(noni), the DNA fingerprints of 13 noni germplasm resources of were studied by ISSR markers. The results showed that ten ISSR primers could amplify 183 bands, of which 159 bands were polymorphic, accounting for 86.9%. The genetic similarity coefifcients of 13 noni germplasm resources ranged from 0.464 to 0.784. Cluster analysis showed that 13 noni germplasm resources could be divided into two clads, in which Small fruit as a single group had distant relation with other germplasm resources. Although all noni germplasm resources could not completely separated according external morphological characteristics, the most with the same characteristics could be clustered into the same group or sub-group.