黑龙江医药
黑龍江醫藥
흑룡강의약
HEILONGJIANG MEDICAL JOURNAL
2014年
6期
1281-1282,1283
,共3页
鲁斯可皂苷元%麦冬%生麦饮(党参方)HPLC-ELSD
魯斯可皂苷元%麥鼕%生麥飲(黨參方)HPLC-ELSD
로사가조감원%맥동%생맥음(당삼방)HPLC-ELSD
Ruscogenin%Ophiopogon japonicus%Shengmaiyin%HPLC-ELSD
目的：建立高效液相色谱法测定生麦饮（党参方）中鲁斯可皂苷元的含量。方法：以Diamonsil(5μm，4.6 mm×250mm)为色谱柱，乙腈-0.2%冰乙酸溶液（25：75）为流动相，流速1.0mL·min-1，柱温30℃，用蒸发光散射检测器检测。结果：鲁斯可皂苷元浓度在6.28～113.00μg/mL范围内浓度对数值与峰面积的对数值呈良好线性关系，r=0.9996，样品平均加样回收率为98.7%(n=6)，RSD为1.2%。结论：本方法简便、准确、重复性好，可用于生麦饮（党参方）中鲁斯可皂苷元的含量测定。
目的：建立高效液相色譜法測定生麥飲（黨參方）中魯斯可皂苷元的含量。方法：以Diamonsil(5μm，4.6 mm×250mm)為色譜柱，乙腈-0.2%冰乙痠溶液（25：75）為流動相，流速1.0mL·min-1，柱溫30℃，用蒸髮光散射檢測器檢測。結果：魯斯可皂苷元濃度在6.28～113.00μg/mL範圍內濃度對數值與峰麵積的對數值呈良好線性關繫，r=0.9996，樣品平均加樣迴收率為98.7%(n=6)，RSD為1.2%。結論：本方法簡便、準確、重複性好，可用于生麥飲（黨參方）中魯斯可皂苷元的含量測定。
목적：건립고효액상색보법측정생맥음（당삼방）중로사가조감원적함량。방법：이Diamonsil(5μm，4.6 mm×250mm)위색보주，을정-0.2%빙을산용액（25：75）위류동상，류속1.0mL·min-1，주온30℃，용증발광산사검측기검측。결과：로사가조감원농도재6.28～113.00μg/mL범위내농도대수치여봉면적적대수치정량호선성관계，r=0.9996，양품평균가양회수솔위98.7%(n=6)，RSD위1.2%。결론：본방법간편、준학、중복성호，가용우생맥음（당삼방）중로사가조감원적함량측정。
Objective:To establish a method for content determination of Ruscogenin in Shengmaiyin by high-performance liquid chromatography (HPLC). Methods: Content determination was carried out on a Diamonsil (5 μm,4.6 mm × 250mm) with acetoni-trile-0.2%glacial acetic acid (25:75) as mobile phase, the flow rate was 1.0 mL·min-1 and column temperature was 30℃. The detec-tion was performed on an evaporative light-scattering detector. Results:Logarithm of Concentration and logarithm of peak area showed good linear relationship when Ruscogenin was in the range of 6.28~113.00μg/mL (r=0.9996), sample average recovery was 98.7%(n=6) with a RSD of 1.2%. Conclusion: This method is simple, accurate and reproducible, it can be used for the content determina-tion of Ruscogenin in Shengmaiyin.
