CAJ | 학술논문

目的：探索糖尿病是否可以通过心肌组织肌浆网钙转运三磷酸腺苷(ATP)酶（SERCA2a）的小泛素样修饰进而影响SERCA2a的活性与表达。 　　方法：采用小动物超声和左心室压力测定评价2型糖尿病大鼠（糖尿病组）和对照大鼠（对照组）心肌收缩和舒张功能；比较糖尿病组大鼠和对照组大鼠心肌组织内SERCA2a的表达情况，用免疫共沉淀方法以及小泛素样修饰试剂盒比较糖尿病组大鼠和对照组大鼠心肌组织内SERCA2a小泛素样修饰程度。 　　结果：和对照组大鼠比较：糖尿病组大鼠心肌收缩、舒张功能均下降，尤以舒张功能下降更为明显；糖尿病组大鼠心肌组织内SERCA2a蛋白水平、基因表达和小泛素样修饰程度均下降；小泛素样修饰E2（Ubc9）的蛋白水平在糖尿病大鼠心肌中也下降，而小泛素样蛋白1（SUMO1）和小泛素样修饰E1（SAE1和SAE2组成的二聚体）蛋白水平无明显变化。 　　结论：SERCA2a小泛素样修饰程度和Ubc9蛋白水平在糖尿病心肌组织中下降，提示SERCA2a小泛素样修饰和Ubc9与糖尿病的心肌损伤密切相关。
목적：탐색당뇨병시부가이통과심기조직기장망개전운삼린산선감(ATP)매（SERCA2a）적소범소양수식진이영향SERCA2a적활성여표체。 　　방법：채용소동물초성화좌심실압력측정평개2형당뇨병대서（당뇨병조）화대조대서（대조조）심기수축화서장공능；비교당뇨병조대서화대조조대서심기조직내SERCA2a적표체정황，용면역공침정방법이급소범소양수식시제합비교당뇨병조대서화대조조대서심기조직내SERCA2a소범소양수식정도。 　　결과：화대조조대서비교：당뇨병조대서심기수축、서장공능균하강，우이서장공능하강경위명현；당뇨병조대서심기조직내SERCA2a단백수평、기인표체화소범소양수식정도균하강；소범소양수식E2（Ubc9）적단백수평재당뇨병대서심기중야하강，이소범소양단백1（SUMO1）화소범소양수식E1（SAE1화SAE2조성적이취체）단백수평무명현변화。 　　결론：SERCA2a소범소양수식정도화Ubc9단백수평재당뇨병심기조직중하강，제시SERCA2a소범소양수식화Ubc9여당뇨병적심기손상밀절상관。
Objective: To investigate the effect of diabetes on the intensity of sarcoplasmic reticulum Ca2+-ATPase (SERCA2a)-SUMOylation and SERCA2a activity of myocardium in experimental rats. Methods: The 8 weeks old SD rats were divided into 2 groups, Diabetic group, with diet-induced type 2 diabetic rats and Control group, with normal rats. The systolic and diastolic cardiac functions were evaluated by echocardiography and left ventricular pressure measurement. The intensity of SERCA2a-SUMOylation was examined by co-immunoprecipitation and SUMOylation kit. Results: Compared with Control group, Diabetic group had decreased systolic and diastolic cardiac functions, especially for diastolic function;decreased SERCA2a protein expression and intensity of SUMOylation;decreased SUMOylation E2 (Ubc9 ) protein expression. The protein levels of SUMO1, SAE1 and SAE2 were similar between 2 groups. Conclusion: The intensity of SERCA2a-SUMOylation and Ubc9 decreased in diabetic myocardium which implies that SERCA2a-SUMOylation and Ubc9 were closely related to the damage of diabetic myocardium in experimental rats.