CAJ | 학술논문

目的：检测锌指蛋白转录因子5（ KLF5）在肾癌组织中的表达，探讨其临床意义。方法选择2009年5月—2012年10月南昌大学附属赣州医院收治的肾癌患者50例，收集所有患者手术切除的肾癌组织及其中15例患者的癌旁组织，采用免疫组织化学S－P法检测KLF5的表达，并分析其与肾癌患者临床病理特征的关系。结果肾癌组织和癌旁组织均有一定水平的KLF5表达，肾癌组织KLF5阳性表达率〔84.0％（42／50）〕高于癌旁组织〔26.7％（4／15）， P＝0.000〕。不同性别、年龄、肿瘤直径肾癌患者KLF5阳性表达率比较，差异无统计学意义（ P＞0.05）。不同组织学分型、组织学分级肾癌患者KLF5表达强度比较，差异均无统计学意义（ P＞0.05）；不同临床分期及有无淋巴转移肾癌患者KLF5表达强度比较，差异均有统计学意义（P＜0.05）。结论 KLF5阳性表达率在肾癌组织中明显升高，其表达强度与肾癌临床分期及淋巴结转移密切相关，或许可以成为判断肾癌发生、发展、预后的一个理想指标。
목적：검측자지단백전록인자5（ KLF5）재신암조직중적표체，탐토기림상의의。방법선택2009년5월—2012년10월남창대학부속공주의원수치적신암환자50례，수집소유환자수술절제적신암조직급기중15례환자적암방조직，채용면역조직화학S－P법검측KLF5적표체，병분석기여신암환자림상병리특정적관계。결과신암조직화암방조직균유일정수평적KLF5표체，신암조직KLF5양성표체솔〔84.0％（42／50）〕고우암방조직〔26.7％（4／15）， P＝0.000〕。불동성별、년령、종류직경신암환자KLF5양성표체솔비교，차이무통계학의의（ P＞0.05）。불동조직학분형、조직학분급신암환자KLF5표체강도비교，차이균무통계학의의（ P＞0.05）；불동림상분기급유무림파전이신암환자KLF5표체강도비교，차이균유통계학의의（P＜0.05）。결론 KLF5양성표체솔재신암조직중명현승고，기표체강도여신암림상분기급림파결전이밀절상관，혹허가이성위판단신암발생、발전、예후적일개이상지표。
Objective To detect the expression of KLF 5 in renal carcinoma , to explore its clinical significance . Methods A total of 50 renal carcinoma patients were selected in Ganzhou Affiliated Hospital of Nanchang University between May 2009 and October 2012.Renal carcinoma tissues of the 50 cases and adjacent tissues of 15 cases out of the 50 cases were collectedafter operation.Immunohistochemical S -P method (two -step) was used to detect the expression of KLF5, and its correlationto the patients′clinical pathological characteristics was analyzed .Results KLF5 expressed in both renal carcinoma tissuesand adjacent tissues.The positive expression rate of KLF5 of renal carcinoma tissues 〔84.0% (42/50)〕was higher than that ofadjacent tissues 〔26.7% (4/15), P =0.000〕.There was no significant differences of positive KLF5 expression rate in renalcarcinoma patients with different gender , ages or tumor diameters (P >0.05), and so as of KLF5 expression intensity in renalcarcinoma patients with different histological classifications or histological differentiations (P >0.05).There were significantdifferences of KLF5 expression intensity in renal carcinoma patients with different clinical stages , with lymphatic metastasis or not(P <0.05).Conclusion The expression of KLF5 in renal carcinoma is higher, and its expression intensity is correlated withclinical stages and lymphatic metastasis, which is probably be used as an ideal biomarker for judging onset , deterioration andprognosis of renal carcinoma.