CAJ | 학술논문

目的：探讨类叶升麻苷（acteoside，AS）对阿尔采末病（Alzheimer’s disease，AD）小鼠皮层组织中 Caspase-3基因表达的影响。方法将昆明（kunming，KM）小鼠随机分为正常组，模型组，vitamin E（VitE）组，类叶升麻苷低、中、高剂量组。除正常组外，其余各组小鼠均腹腔注射60 mg·kg －1· d －1的 D-半乳糖和灌胃5 mg·kg －1·d －1的三氯化铝，连续造模60 d 以制备 AD 模型。然后给以30、60、120 mg·kg －1·d －1的 AS 治疗30 d，期间造模继续。给药完成后，利用跳台法测定小鼠的学习和记忆能力，化学比色法测定小鼠血清及脑组织中的 AChE 活性；HE 染色观察各组小鼠皮层组织结构变化；免疫组化分析小鼠皮层组织中 caspase-3基因表达的变化。结果与模型组相比，AS 给药组小鼠的学习记忆能力有所改善，其下台潜伏期和错误次数均明显延长和减少（P ＜0.05或 P ＜0.01），血清和脑组织中 AChE 活性明显降低（P ＜0.05或 P ＜0.01），皮层组织中神经细胞的形态和数量明显改善（P ＜0.01），且皮层组织中 caspase-3基因表达明显下调（P ＜0.05或 P ＜0.01）。结论AS 对 D-半乳糖联合三氯化铝诱导的小鼠脑损伤具有明显保护作用，其保护机制可能是通过抑制小鼠皮层组织 caspase-3基因表达，进而维持皮层组织神经细胞的正常形态及数量。
목적：탐토류협승마감（acteoside，AS）대아이채말병（Alzheimer’s disease，AD）소서피층조직중 Caspase-3기인표체적영향。방법장곤명（kunming，KM）소서수궤분위정상조，모형조，vitamin E（VitE）조，류협승마감저、중、고제량조。제정상조외，기여각조소서균복강주사60 mg·kg －1· d －1적 D-반유당화관위5 mg·kg －1·d －1적삼록화려，련속조모60 d 이제비 AD 모형。연후급이30、60、120 mg·kg －1·d －1적 AS 치료30 d，기간조모계속。급약완성후，이용도태법측정소서적학습화기억능력，화학비색법측정소서혈청급뇌조직중적 AChE 활성；HE 염색관찰각조소서피층조직결구변화；면역조화분석소서피층조직중 caspase-3기인표체적변화。결과여모형조상비，AS 급약조소서적학습기억능력유소개선，기하태잠복기화착오차수균명현연장화감소（P ＜0.05혹 P ＜0.01），혈청화뇌조직중 AChE 활성명현강저（P ＜0.05혹 P ＜0.01），피층조직중신경세포적형태화수량명현개선（P ＜0.01），차피층조직중 caspase-3기인표체명현하조（P ＜0.05혹 P ＜0.01）。결론AS 대 D-반유당연합삼록화려유도적소서뇌손상구유명현보호작용，기보호궤제가능시통과억제소서피층조직 caspase-3기인표체，진이유지피층조직신경세포적정상형태급수량。
Aim To investigate the effect of acteoside (AS)on the expression of caspase-3 in cerebral cortex of mouse models of Alzheimer’s disease(AD).Meth-ods Kunming (KM)strain mice were assigned into control group,model group,positive control group (VitE)and acteoside group.Every group was induced by a combination of D-galactose(i.p.60mg·kg -1 · d -1 )and AlCl3 (i.g.5mg·kg -1 ·d -1 )for 60ds ex-cept for control group,then mice were treated by dif-ferent concentrations(30,60,1 20 mg·kg -1 ·d -1 )of acteoside for 30ds.During the time,mice were in-duced continuously by a combination of D-galactose and AlCl3 .The learning and memory of mice were de-tected by step-down test,the activity of AChE in serum and brain of mice was measured by chemical colorime-try,the structure changes in cerebral cortex were ob-served by HE staining,and the expression of caspase-3 in cerebral cortex was analyzed through the immunohis-tochemical staining.Results Compared with model group,acteoside could improve the learning and mem-ory abilities(P <0.05 or P <0.01 ),decrease the ac-tivity of AChE in serum and brain(P <0.05 or P <0.01 ),and improve the morphology and number of neuron in cerebral cortex(P <0.01 ).Moreover,acte-oside could significantly inhibit the expression of caspase-3 in cerebral cortex (P <0.05,P <0.01 ). Conclusion Acteoside has significantly protective effects on brain damage of mice induced by a combina-tion of D-galactose and AlCl3 , and it′s protective mechanism probably relate to inhibiting the expression of caspase-3 and maintainings the normal morphology and number of neuron in cerebral cortex.