CAJ | 학술논문

目的：研究5种黄连生物碱（小檗碱、黄连碱、巴马汀、表小檗碱、药根碱）对小鼠睡眠的影响，初步探讨其作用机制。方法通过小鼠自主活动实验和阈上剂量、阈下剂量戊巴比妥钠诱导小鼠睡眠实验，观察药物对小鼠入睡行为的影响。同时建立 PCPA 小鼠失眠模型，利用高效液相－荧光检测器，检测其下丘脑中神经递质 NE、DA 和5-HT 的含量。结果与对照组相比，在行为学实验中 ig 75 mg·kg －1·d －1的小檗碱和黄连碱能明显抑制小鼠的自主活动能力，且增加阈下剂量戊巴比妥钠小鼠入睡百分率。与模型组相比，小檗碱和黄连碱的 NE 和5-HT 含量明显增加（P ＜0.01），DA 含量无影响（P ＞0.05）。结论小檗碱和黄连碱可能通过增加PCPA 失眠模型小鼠下丘脑中5-HT 和 NE 含量，来发挥一定的镇静、催眠的作用，且小檗碱作用强于黄连碱。其余各生物碱对小鼠睡眠无影响。
목적：연구5충황련생물감（소벽감、황련감、파마정、표소벽감、약근감）대소서수면적영향，초보탐토기작용궤제。방법통과소서자주활동실험화역상제량、역하제량무파비타납유도소서수면실험，관찰약물대소서입수행위적영향。동시건립 PCPA 소서실면모형，이용고효액상－형광검측기，검측기하구뇌중신경체질 NE、DA 화5-HT 적함량。결과여대조조상비，재행위학실험중 ig 75 mg·kg －1·d －1적소벽감화황련감능명현억제소서적자주활동능력，차증가역하제량무파비타납소서입수백분솔。여모형조상비，소벽감화황련감적 NE 화5-HT 함량명현증가（P ＜0.01），DA 함량무영향（P ＞0.05）。결론소벽감화황련감가능통과증가PCPA 실면모형소서하구뇌중5-HT 화 NE 함량，래발휘일정적진정、최면적작용，차소벽감작용강우황련감。기여각생물감대소서수면무영향。
Aim To study the hypnotic effect of five alkaloids extracted from Rhizoma Coptidis (berberine , coptisine,palmatine,epiberberine,jatrorrhizine )in mice,and preliminarily explore its underlying mecha-nism.Methods The experiments of locomotor activity and hypnosis induced by suprathreshold and subthresh-old doses of pentobarbital sodium were used to evaluate the effect of drugs on sleep behavior in mice.Then, HPLC-FLD was used to detect the contents of NE,DA and 5-HT on PCPA mice model.Results Compared with control group,berberine and coptisine notably in-hibited spontaneous activity in behavioral experiments (P <0.05),and increased the sleeping percentage of mice under subthreshold dose of pentobarbital sodium. Berberine and coptisine shortened the period of sleep latency,and prolonged the sustained period of sleeping at suprathreshold dose in mice (P <0.05 or P <0.01 ).Other alkaloids had no significant differences in sleep latency and period of sleep observed in this current experiment.Compared with PCPA mice model group,berberine and coptisine remarkably increased the contents of NE and 5-HT (P <0.01 ),but they had no effects on DA.Conclusions Berberine and coptisine may play a sedative and hypnotic role in PC-PA mice by increasing contents of 5-HT and NE in hy-pothalamus,and the sedative and hypnotic effects of berberine are stronger than those of coptisine.Other alkaloids have no effects on sleeping in mice.