CAJ | 학술논문

目的：探讨 BNIP3蛋白表达在肿瘤低氧环境中对胃癌的作用及探讨其影响自噬水平的机制。方法胃癌手术标本取材18例，分别通过免疫组化和免疫印迹检测 BNIP3和LC3的蛋白表达水平在癌组织和正常组织中的差异；培养中分化的人胃癌细胞系 SGC-7901，在氯化钴（CoCl2）引发的低氧环境中，观察 BNIP3 siRNA 干扰后对自噬相关蛋白 LC3的表达变化，以及使用自噬阻滞剂3-甲基腺嘌呤（3-methylade-nine，3-MA）后对细胞活力的影响。结果免疫组化和免疫印迹检测中均可见肿瘤组织 BNIP3蛋白表达量较正常组织增高，同时肿瘤组织中 LC3-II 表达水平也明显上调。BNIP3 siRNA 使胃癌细胞 SGC-7901中 LC3-Ⅱ蛋白表达下调，而且自噬水平降低后，在低氧环境中细胞活力明显提高。结论BNIP3蛋白能够通过调控自噬水平对胃癌细胞在乏氧环境中细胞死亡发挥重要的作用。
목적：탐토 BNIP3단백표체재종류저양배경중대위암적작용급탐토기영향자서수평적궤제。방법위암수술표본취재18례，분별통과면역조화화면역인적검측 BNIP3화LC3적단백표체수평재암조직화정상조직중적차이；배양중분화적인위암세포계 SGC-7901，재록화고（CoCl2）인발적저양배경중，관찰 BNIP3 siRNA 간우후대자서상관단백 LC3적표체변화，이급사용자서조체제3-갑기선표령（3-methylade-nine，3-MA）후대세포활력적영향。결과면역조화화면역인적검측중균가견종류조직 BNIP3단백표체량교정상조직증고，동시종류조직중 LC3-II 표체수평야명현상조。BNIP3 siRNA 사위암세포 SGC-7901중 LC3-Ⅱ단백표체하조，이차자서수평강저후，재저양배경중세포활력명현제고。결론BNIP3단백능구통과조공자서수평대위암세포재핍양배경중세포사망발휘중요적작용。
Aim To study the effect of BNIP3 on hy-poxia-induced autophagic cell death in gastric carcino-ma.Methods The protein levels of BNIP3 and LC3-Ⅱ of 1 8 cases of gastric carcinoma were studied with immunocytochemistry and Western blot analysis be-tween normal and tumor tissues.The expression of LC3-Ⅱ in SGC-7901 cells was assessed while knock-down of BNIP3 by siRNA in CoCl2-induced hypoxia, and the effect of 3-MA was also studied.Cell viability in hypoxia treatment for 48 h was estimated with cell counting kit-8 (CCK8).Results Compared with nor-mal tissues,the protein levels of BNIP3 and LC3-Ⅱwere increased in tumor tissues.BNIP3 siRNA could decrease the expression of LC3-Ⅱ and enhance the cell viability,while inhibition of autophagy could also in-crease the cell viability in SGC-7901 cells.Conclu-sions BNIP3 may play a role in hypoxia-induced cell death in gastric carcinoma,and the possible mecha-nism may be related to the regulation of autophagy.