CAJ | 학술논문

目的：研究山茱萸活性成分马钱苷、莫诺苷对 D-半乳糖致衰大鼠星形胶质细胞的保护作用。方法常规方法体外培养乳大鼠大脑皮层星形胶质细胞，采用免疫荧光方法进行星形胶质细胞鉴定；MTT 法对比不同浓度 D-半乳糖对星形胶质细胞的增殖抑制作用，以及马钱苷、莫诺苷对 D-半乳糖致衰星形胶质细胞活力的影响；以超氧化物歧化酶（SOD）、丙二醛（MDA）为观察指标，观察马钱苷、莫诺苷对D-半乳糖致衰星形胶质细胞的影响；ELISA 法检测胶质细胞源性神经营养因子（GDNF）、碱性成纤维细胞生长因子（bF-GF）含量变化；Western blot 法检测 Bax、caspase-3、磷酸化细胞外信号调节激酶（p-ERK1／2）、磷酸化丝裂原活化蛋白激酶／ERK 激酶（p-MEK1／2）的表达水平，以做进一步机制研究。结果马钱苷、莫诺苷组与模型组比较，细胞的增殖能力提高，SOD 含量增加，MDA 活性降低，且差异有显著性（P＜0.05）；马钱苷、莫诺苷组生长因子（GDNF、bFGF）的分泌高于模型组；Western blot 结果表明，D-半乳糖致衰老的星形胶质细胞中 Bax、caspase-3蛋白表达无明显变化，模型组 p-ERK1／2、p-MEK1／2蛋白表达降低，马钱苷、莫诺苷增加 p-ERK1／2、p-MEK1／2蛋白的表达。结论山茱萸活性成分马钱苷、莫诺苷可通过提高 D-半乳糖致衰星形胶质细胞的增殖能力，提高衰老细胞的抗氧化能力，发挥抗衰老作用，其机制可能与 MEK／ERK 介导的信号通路调控有关。
목적：연구산수유활성성분마전감、막낙감대 D-반유당치쇠대서성형효질세포적보호작용。방법상규방법체외배양유대서대뇌피층성형효질세포，채용면역형광방법진행성형효질세포감정；MTT 법대비불동농도 D-반유당대성형효질세포적증식억제작용，이급마전감、막낙감대 D-반유당치쇠성형효질세포활력적영향；이초양화물기화매（SOD）、병이철（MDA）위관찰지표，관찰마전감、막낙감대D-반유당치쇠성형효질세포적영향；ELISA 법검측효질세포원성신경영양인자（GDNF）、감성성섬유세포생장인자（bF-GF）함량변화；Western blot 법검측 Bax、caspase-3、린산화세포외신호조절격매（p-ERK1／2）、린산화사렬원활화단백격매／ERK 격매（p-MEK1／2）적표체수평，이주진일보궤제연구。결과마전감、막낙감조여모형조비교，세포적증식능력제고，SOD 함량증가，MDA 활성강저，차차이유현저성（P＜0.05）；마전감、막낙감조생장인자（GDNF、bFGF）적분비고우모형조；Western blot 결과표명，D-반유당치쇠로적성형효질세포중 Bax、caspase-3단백표체무명현변화，모형조 p-ERK1／2、p-MEK1／2단백표체강저，마전감、막낙감증가 p-ERK1／2、p-MEK1／2단백적표체。결론산수유활성성분마전감、막낙감가통과제고 D-반유당치쇠성형효질세포적증식능력，제고쇠로세포적항양화능력，발휘항쇠로작용，기궤제가능여 MEK／ERK 개도적신호통로조공유관。
Aim To study effects of loganin and morro-niside on aging astrocytes induced by D-galactose. Methods Cortex astrocytes of newly born rats were cultured in vivo and indentified by immunofluorescence method.Firstly,appropriate D-galactose concentration was selected and effects of loganin and morroniside on proliferation activity of aging astrocytes induced by D-galactose were determined by MTT test.Then SOD, MDA were taken as indicators to study the effects of loganin and morroniside on aging astrocytes induced by D-galactose.Thirdly,growth factors like gliar cell line-derived neurotrophic factor (GDNF),basic fibro-blast growth factor (bFGF)tested by ELISA method and expression of Bax,caspase-3,phospho-extracellu-lar signal-regulated kinese (p-ERK1 /2),phospho-mi-togen-actived protein kinase /extracellular signal-regu-lated kinase kinase (p-MEK1 /2)were taken as indi-cators to discuss the potential protection mechanism. Results Loganin and morroniside exerted certain effects on proliferation of aging astrocytes induced by D-galactose,improving SOD,GDNF,bFGF release and lowering MDA release significantly (P <0.05 ). The expressions of Bax and caspase-3 proteins had no difference between model group and loganin group, morroniside group.While the expressions of p-ERK1 /2,p-MEK1 /2 of loganin group,morroniside group were improved significantly compared with model group.Conclusion Loganin and morroniside have protective effects on aging astrocytes induced by D-ga-lactose,and increase the proliferation ability.Protec-ting their antioxidant systems and improving the expres-sion of p-ERK1 /2,p-MEK1 /2 proteins are possible mechanisms.