中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2014年
12期
1671-1676
,共6页
孟龙%王海波%邓志钦%汪源%伍嘉宝%赖周毅%吕瑞玲%孙晓雪%朱林燕%陈丽新%王立伟
孟龍%王海波%鄧誌欽%汪源%伍嘉寶%賴週毅%呂瑞玲%孫曉雪%硃林燕%陳麗新%王立偉
맹룡%왕해파%산지흠%왕원%오가보%뢰주의%려서령%손효설%주림연%진려신%왕립위
冰片%氯通道%氯通道阻断剂%癌细胞%膜片钳技术%细胞体积
冰片%氯通道%氯通道阻斷劑%癌細胞%膜片鉗技術%細胞體積
빙편%록통도%록통도조단제%암세포%막편겸기술%세포체적
Borneol%chloride channels%chloride channel blocker%tumor cells%patch clamp technique%cell volume
目的：探讨冰片对低分化鼻咽癌（CNE-2Z）细胞氯通道的激活作用及对细胞容积的影响。方法采用全细胞膜片钳技术记录冰片激活 CNE-2Z 细胞膜电流，分析其电流特性；用活细胞图像法观察并测量冰片对细胞体积的影响。结果细胞外灌流冰片（20μmol· L －1）诱导 CNE-2Z 细胞膜氯通道开放，产生氯电流，该电流有明显外向优势，无电压及时间依赖性失活，其翻转电位接近氯离子平衡电位，该电流可被氯通道阻断剂 tamoxifen 抑制，细胞外灌流47％高渗溶液完全抑制该电流。细胞外灌流冰片30 min，细胞体积缩小约9.4％，该作用可被 tamoxifen 完全抑制。结论冰片可以激活低分化鼻咽癌细胞容积敏感性氯通道，导致细胞体积缩小，氯通道在冰片引起细胞体积缩小中起着重要作用。
目的：探討冰片對低分化鼻嚥癌（CNE-2Z）細胞氯通道的激活作用及對細胞容積的影響。方法採用全細胞膜片鉗技術記錄冰片激活 CNE-2Z 細胞膜電流，分析其電流特性；用活細胞圖像法觀察併測量冰片對細胞體積的影響。結果細胞外灌流冰片（20μmol· L －1）誘導 CNE-2Z 細胞膜氯通道開放，產生氯電流，該電流有明顯外嚮優勢，無電壓及時間依賴性失活，其翻轉電位接近氯離子平衡電位，該電流可被氯通道阻斷劑 tamoxifen 抑製，細胞外灌流47％高滲溶液完全抑製該電流。細胞外灌流冰片30 min，細胞體積縮小約9.4％，該作用可被 tamoxifen 完全抑製。結論冰片可以激活低分化鼻嚥癌細胞容積敏感性氯通道，導緻細胞體積縮小，氯通道在冰片引起細胞體積縮小中起著重要作用。
목적：탐토빙편대저분화비인암（CNE-2Z）세포록통도적격활작용급대세포용적적영향。방법채용전세포막편겸기술기록빙편격활 CNE-2Z 세포막전류，분석기전류특성；용활세포도상법관찰병측량빙편대세포체적적영향。결과세포외관류빙편（20μmol· L －1）유도 CNE-2Z 세포막록통도개방，산생록전류，해전류유명현외향우세，무전압급시간의뢰성실활，기번전전위접근록리자평형전위，해전류가피록통도조단제 tamoxifen 억제，세포외관류47％고삼용액완전억제해전류。세포외관류빙편30 min，세포체적축소약9.4％，해작용가피 tamoxifen 완전억제。결론빙편가이격활저분화비인암세포용적민감성록통도，도치세포체적축소，록통도재빙편인기세포체적축소중기착중요작용。
Aim To clarify the effect of Borneol on the chloride channels and cell volume in poorly differentia-ted nasopharyngeal carcinoma CNE-2Z cells.Methods The technique of whole-cell patch clamp was used to detect the chloride currents and analyze the character-istics of the currents in CNE-2Z cells.The volume changes caused by Borneol were measured by the meth-od of time-lapse live cell imaging.Results The chlo-ride currents were induced by extracellular application of Borneol (20 μmol·L -1 )isotonic condition.The currents showed a characteristic of outward rectification and did not show voltage-dependent or time-dependent inactivation.The reversal potential of the currents was close to the CI-equilibrium potential. The currents were inhibited by the chloride channel blocker tamox-ifen.The currents were also inhibited by 47% hyper-tonic solution.Borneol decreased the cell volume by 9.4% in 30 min.Tamoxifen completely inhibited the Borneol-induced cell volume decrease.Conclusion Borneol can activate volume-sensitive chloride channels and induce volume decrease in CNE-2Z cells.Chloride channels play a pivotal role in the process of volume decrease caused by Borneol.
