CAJ | 학술논문

目的：检测子宫内膜异位症（EMs）患者子宫内膜间质细胞中环氧化酶-2（COX-2）、前列腺素E2（PGE2）、锌指转录因子（Snail）和E-钙黏蛋白（E-cadherin）mRNA表达情况，分析EMs中子宫内膜间质细胞的侵袭、转移能力。方法收集EMs患者的在位及异位病灶子宫内膜组织和子宫肌瘤患者的在位内膜组织，原代消化培养子宫内膜细胞，取纯化的第4代子宫内膜间质细胞进行研究，RT-PCR法检测细胞COX-2、PGE2、Snail和E-cad?herin mRNA的表达。结果 EMs和子宫肌瘤患者分泌期在位子宫内膜间质细胞COX-2、PGE2、Snail和E-cadherin mRNA表达与增殖期比较差异均无统计学意义；EMs患者在位及异位的子宫内膜间质细胞COX-2、PGE2、Snail mRNA表达比肌瘤患者在位细胞均明显升高，而E-cadherin mRNA表达降低（P＜0.05或P＜0.01）；EMs患者异位的子宫内膜间质细胞COX-2、PGE2、Snail mRNA表达比在位间质细胞升高，而E-cadherin mRNA表达降低（P＜0.05或P＜0.01）。结论 EMs患者的子宫内膜间质细胞侵袭、转移能力强于非EMs患者，且在EMs发病中异位病灶子宫内膜间质细胞的侵袭、转移能力更强。
목적：검측자궁내막이위증（EMs）환자자궁내막간질세포중배양화매-2（COX-2）、전렬선소E2（PGE2）、자지전록인자（Snail）화E-개점단백（E-cadherin）mRNA표체정황，분석EMs중자궁내막간질세포적침습、전이능력。방법수집EMs환자적재위급이위병조자궁내막조직화자궁기류환자적재위내막조직，원대소화배양자궁내막세포，취순화적제4대자궁내막간질세포진행연구，RT-PCR법검측세포COX-2、PGE2、Snail화E-cad?herin mRNA적표체。결과 EMs화자궁기류환자분비기재위자궁내막간질세포COX-2、PGE2、Snail화E-cadherin mRNA표체여증식기비교차이균무통계학의의；EMs환자재위급이위적자궁내막간질세포COX-2、PGE2、Snail mRNA표체비기류환자재위세포균명현승고，이E-cadherin mRNA표체강저（P＜0.05혹P＜0.01）；EMs환자이위적자궁내막간질세포COX-2、PGE2、Snail mRNA표체비재위간질세포승고，이E-cadherin mRNA표체강저（P＜0.05혹P＜0.01）。결론 EMs환자적자궁내막간질세포침습、전이능력강우비EMs환자，차재EMs발병중이위병조자궁내막간질세포적침습、전이능력경강。
Objective To analyze the invasion and metastasis of endometrial stromal cells in endometriosis through assessing the transcription levels of COX-2, PGE2, Snail and E-cadherin mRNA. Methods Ectopic and eutopic endometri?al tissues from patients with EMs (endometriosis) or uterine fibroids were collected and cultured. The fourth generation of pu?rified endometrial stromal cells were used for research, and the transcription levels of COX-2, PGE2, Snail and E-cadherin mRNA were analyzed by RT-PCR. Results There were no statistical differences in transcription levels of COX-2, PGE2, Snail and E-cadherin mRNA in eutopic endometrial stromal cells from patients with EMs or uterine fibroids between secra?tion stage and proliferation stage(P>0.05). The transcription levels of COX-2, PGE2 and Snail mRNA were significantly in?creased while transcription level of E-cadherin mRNA was decreased in eutopic and ectopic endometrial stromal cells in pa?tients with EM compared with those in patients with uterine fibroid. And the difference was statistically significant(P<0.05 or P<0.01). The transcription levels of COX-2, PGE2 and Snail mRNA were obviously higher while transcription level of E-cadherin mRNA was lower in ectopic endometrial stromal cells than those in eutopic endometrial stromal cells from pa?tients with EMs. And the difference was statistically significant(P<0.05 or P<0.01). Conclusion The invasion and me?tastasis of endometrial stromal cells of patients with EMs were remarkable than those cell of patients without EMs. What ’s more, the invasion and metastasis in ectopic endometrial stromal cells were more obvious than those cells in the pathogenesis of EMs.