天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2014年
12期
1202-1204,1253
,共4页
肾透明细胞癌%Hedgehog信号通路%免疫组织化学%印迹法,蛋白质
腎透明細胞癌%Hedgehog信號通路%免疫組織化學%印跡法,蛋白質
신투명세포암%Hedgehog신호통로%면역조직화학%인적법,단백질
renal clear cell carcinoma%Hedgehog signaling pathway%immunohistochemistry%blotting,Western
目的：研究人肾透明细胞癌（RCCC）中Hedgehog信号通路相关因子Shh、Ptch、Smo和Gli1蛋白的表达，探讨Hedgehog信号转导通路的异常激活与RCCC的关系。方法应用免疫组织化学方法检测76例RCCC标本及12例癌旁正常肾组织，应用蛋白免疫印迹技术检测10例新鲜癌组织及癌旁正常肾组织中Shh、Ptch、Smo和Gli1蛋白的表达。结果 Shh、Ptch、Smo和Gli1蛋白在RCCC中的异常表达率（分别为52.6%、63.2%、44.7%、60.5%）高于正常肾组织(分别为8.3%、16.7%、8.3%、25.0%)。且其表达与患者的年龄、性别和肿瘤大小无显著相关，而与肿瘤临床分期有关，随肿瘤的恶性程度加重，表达率增加。结论 Hedgehog信号转导通路在RCCC中呈现高度激活状态，此通路的激活可能与RCCC的发生、发展密切相关，有望成为治疗RCCC的靶点。
目的：研究人腎透明細胞癌（RCCC）中Hedgehog信號通路相關因子Shh、Ptch、Smo和Gli1蛋白的錶達，探討Hedgehog信號轉導通路的異常激活與RCCC的關繫。方法應用免疫組織化學方法檢測76例RCCC標本及12例癌徬正常腎組織，應用蛋白免疫印跡技術檢測10例新鮮癌組織及癌徬正常腎組織中Shh、Ptch、Smo和Gli1蛋白的錶達。結果 Shh、Ptch、Smo和Gli1蛋白在RCCC中的異常錶達率（分彆為52.6%、63.2%、44.7%、60.5%）高于正常腎組織(分彆為8.3%、16.7%、8.3%、25.0%)。且其錶達與患者的年齡、性彆和腫瘤大小無顯著相關，而與腫瘤臨床分期有關，隨腫瘤的噁性程度加重，錶達率增加。結論 Hedgehog信號轉導通路在RCCC中呈現高度激活狀態，此通路的激活可能與RCCC的髮生、髮展密切相關，有望成為治療RCCC的靶點。
목적：연구인신투명세포암（RCCC）중Hedgehog신호통로상관인자Shh、Ptch、Smo화Gli1단백적표체，탐토Hedgehog신호전도통로적이상격활여RCCC적관계。방법응용면역조직화학방법검측76례RCCC표본급12례암방정상신조직，응용단백면역인적기술검측10례신선암조직급암방정상신조직중Shh、Ptch、Smo화Gli1단백적표체。결과 Shh、Ptch、Smo화Gli1단백재RCCC중적이상표체솔（분별위52.6%、63.2%、44.7%、60.5%）고우정상신조직(분별위8.3%、16.7%、8.3%、25.0%)。차기표체여환자적년령、성별화종류대소무현저상관，이여종류림상분기유관，수종류적악성정도가중，표체솔증가。결론 Hedgehog신호전도통로재RCCC중정현고도격활상태，차통로적격활가능여RCCC적발생、발전밀절상관，유망성위치료RCCC적파점。
Objective To investigate the expression of Shh, Ptch, Smo and Gli1 proteins who belongs to components of Hedgehog signaling pathway, and to explore the relationship between the abnormal activated of Hedgehog signaling path?way with renal clear cell carcinoma (RCCC). Methods Protein expressions were examined by immunohistochemistry in 76 cases of human RCCC and 12 pericarcinomatous tissues. And 10 fresh specimens from cancer tissues and pericarcinoma?tous tissues were used to examine expressions of Shh, Ptch, Smo and Gli1 by Western blotting. Results The expressions of examined proteins were higher in RCCC than that in pericarcinomatous tissue. Shh (52.6%vs 8.3%), Ptch (63.2%vs 16.7%), Smo (44.7%vs 8.3%) and Gli1 (60.5%vs 25.0%). The increased proteins levels were disassociated with age, gender, and tu?morous size in RCCC. But it was significantly correlated with TNM stage of RCCC. The expressions were gradually increased with higher TNM stage of RCCC or more invasive malignancy. Conclusion Hedgehog signaling pathway is highly activated in RCCC. It might be involved in initiation, progression of RCCC, and it might be used as the target for RCCC treatment.
