CAJ | 학술논문

目的：探讨泽泻汤加味方对高盐高血压大鼠肾脏水通道蛋白（AQP）-2表达的影响。方法以8%高盐饲料喂养的方法制备高盐高血压动物模型50只，造模成功后随机分为模型组，西药组，中药高、中、低组，各10只；另选10只普通饲料喂养大鼠为正常组。中药高、中、低组分别给予泽泻汤加味方混悬液16.2、10.8、5.4 g/(kg·d)；西药组给予缬沙坦氢氯噻嗪溶液16.65 mg/(kg·d)；模型组及正常组灌服等体积蒸馏水。均按每天1 mL/100 g灌胃，灌胃4周。于给药1、4、7、14、28 d时测定大鼠收缩压（SBP），并收集24 h尿量。用免疫组织化学法和逆转录-聚合酶链反应（RT-PCR）分别检测肾脏AQP-2蛋白和mRNA的表达。结果 SBP由高到低依次是：模型组＞中药中组和中药低组＞西药组和中药高组＞正常组，尿量由高到低依次是：西药组和中药高组＞中药中组和中药低组＞模型组＞正常组，中药中组与中药低组间、西药组与中药高组间差异无统计学意义。模型组肾脏AQP-2沿集合管内壁上皮细胞分布的棕黄色颗粒较正常组及中药高、中、低组明显颜色加深而浓厚，分布面积更广泛。除中药低组AQP-2 mRNA的表达水平与模型组差异无统计学意义外，其余各组均低于模型组，高于中药高组（均P＜0.05）。结论泽泻汤加味方可通过抑制AQP-2的高表达使血压降低。
목적：탐토택사탕가미방대고염고혈압대서신장수통도단백（AQP）-2표체적영향。방법이8%고염사료위양적방법제비고염고혈압동물모형50지，조모성공후수궤분위모형조，서약조，중약고、중、저조，각10지；령선10지보통사료위양대서위정상조。중약고、중、저조분별급여택사탕가미방혼현액16.2、10.8、5.4 g/(kg·d)；서약조급여힐사탄경록새진용액16.65 mg/(kg·d)；모형조급정상조관복등체적증류수。균안매천1 mL/100 g관위，관위4주。우급약1、4、7、14、28 d시측정대서수축압（SBP），병수집24 h뇨량。용면역조직화학법화역전록-취합매련반응（RT-PCR）분별검측신장AQP-2단백화mRNA적표체。결과 SBP유고도저의차시：모형조＞중약중조화중약저조＞서약조화중약고조＞정상조，뇨량유고도저의차시：서약조화중약고조＞중약중조화중약저조＞모형조＞정상조，중약중조여중약저조간、서약조여중약고조간차이무통계학의의。모형조신장AQP-2연집합관내벽상피세포분포적종황색과립교정상조급중약고、중、저조명현안색가심이농후，분포면적경엄범。제중약저조AQP-2 mRNA적표체수평여모형조차이무통계학의의외，기여각조균저우모형조，고우중약고조（균P＜0.05）。결론택사탕가미방가통과억제AQP-2적고표체사혈압강저。
Objective To explore the effect of modified Zexie Decoction on renal aquaporin-2 (AQP2) expression in high-salt hypertensive rat. Methods Hypertensive rats model was established by feeding rat with 8%high salt. Rats (n=50) were divided into model group, modern medicine group, traditional Chinese medicine groups of high, medium, low dose, with 10 rats in each group. The other 10 rats were fed with ordinary diet as normal group. Rats in traditional Chinese medi?cine of high, medium, low groups were given Zexie Decoction suspension of 16.2, 10.8 and 5.4 g/(kg·d) respectively;Rats in modern medicine group was given Valsartan hydrochlorothiazide 16.65 mg/(kg·d);the model group and normal group was ad?ministered with equal volume of distilled water. Animals were feed with medications at 1 mL/100 g by gavage for 4 weeks. On the 1st , 4th , 7th , 14th and, 28th day of administration, we measured SBP and collected 24 h urine. We employed immunohis?tochemistry to detect renal AQP-2 protein expression level and RT-PCR to detect renal AQP-2 mRNA transcription level. Results The rank of SBP from high to low is:model group>traditional Chinese medicine medium and low dose groups>traditional Chinese medicine high dose group and western medicine group>normal group. The rank of urine volume from high to low is:Western medicine group and traditional Chinese medicine high dose group>traditional Chinese medicine me?dium and low dose group > normal group, the difference was not statistically significant between traditional Chinese medi?cine medium and low dose group , or between western medicine group and traditional Chinese medicine high dose group. The renal AQP-2 in epithelial cells along the collecting duct wall of rats in model group show brown particles which are darker and wider distributed than those in normal group and traditional Chinese medicine of high, medium, low dose groups. RT-PCR results show that AQP-2 mRNA expression is highest in rats of model group and lowest in rats of traditional Chi?nese medicine high dose group (P<0.05). No statistical significance of AQP mRNA level was found between traditional Chi?nese medicine low group and model group (P < 0.05). Conclusion Modified Zexie Decoction can lower blood pres?sure by inhibiting the expression of AQP-2.