天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2014年
12期
1172-1175,1251
,共5页
王小琴%彭聿平%刘展%邱一华
王小琴%彭聿平%劉展%邱一華
왕소금%팽율평%류전%구일화
关节炎,类风湿%儿茶酚胺类%T淋巴细胞,调节性%酪氨酸单氧化酶%Th17细胞%胶原诱导性关节炎
關節炎,類風濕%兒茶酚胺類%T淋巴細胞,調節性%酪氨痠單氧化酶%Th17細胞%膠原誘導性關節炎
관절염,류풍습%인다분알류%T림파세포,조절성%락안산단양화매%Th17세포%효원유도성관절염
arthritis,rheumatoid%catecholamines%T-lymphocytes,regulatory%tyrosine 3-monooxygenase%Th17 cells%collagen-induced arthritis
目的：探讨胶原诱导性关节炎（CIA）疾病发生发展过程中Th17和Treg细胞来源的儿茶酚胺（CAs）的作用。方法随机将18只雄性DBA/1小鼠分为对照组、CIA模型Ⅰ组（35 d）和CIA模型Ⅱ组（55 d）。Ⅱ型胶原乳剂尾根部注射制备CIA小鼠模型。Real-time PCR法检测淋巴结中Th17细胞的特异性转录因子ROR-γt，相关细胞因子IL-17和IL-22，Treg细胞的特异性转录因子Foxp3，相关细胞因子转化生长因子（TGF）-β以及酪氨酸羟化酶（TH）的mRNA表达。免疫荧光组织化学染色法观察淋巴结中ROR-γt和Foxp3分别与TH、囊泡单胺转运体-2（VMAT-2）和单胺氧化酶（MAO）的共定位情况。结果与对照组相比，CIA模型Ⅰ组和模型Ⅱ组的小鼠淋巴结中ROR-γ、IL-17、TH和IL-22的mRNA表达增加；Foxp3和TGF-β的mRNA表达减少；其中模型Ⅱ组IL-17的mRNA表达与模型Ⅰ组相比有所降低。对照组和CIA模型Ⅰ组及模型Ⅱ组的小鼠淋巴结中存在ROR-γt和Foxp3分别与TH、VMAT-2和MAO的共定位细胞。CIA模型组小鼠淋巴结中ROR-γt/TH、ROR-γt/VMAT-2和ROR-γt/MAO双阳性细胞数增加，模型Ⅱ组中的这些双阳性细胞数与模型Ⅰ组相比有所减少。结论 CIA模型组小鼠淋巴结中Th17细胞合成CAs的能力增强，这种Th17细胞来源的CAs的增加可能在CIA的发生发展过程有一定的抗炎作用。
目的：探討膠原誘導性關節炎（CIA）疾病髮生髮展過程中Th17和Treg細胞來源的兒茶酚胺（CAs）的作用。方法隨機將18隻雄性DBA/1小鼠分為對照組、CIA模型Ⅰ組（35 d）和CIA模型Ⅱ組（55 d）。Ⅱ型膠原乳劑尾根部註射製備CIA小鼠模型。Real-time PCR法檢測淋巴結中Th17細胞的特異性轉錄因子ROR-γt，相關細胞因子IL-17和IL-22，Treg細胞的特異性轉錄因子Foxp3，相關細胞因子轉化生長因子（TGF）-β以及酪氨痠羥化酶（TH）的mRNA錶達。免疫熒光組織化學染色法觀察淋巴結中ROR-γt和Foxp3分彆與TH、囊泡單胺轉運體-2（VMAT-2）和單胺氧化酶（MAO）的共定位情況。結果與對照組相比，CIA模型Ⅰ組和模型Ⅱ組的小鼠淋巴結中ROR-γ、IL-17、TH和IL-22的mRNA錶達增加；Foxp3和TGF-β的mRNA錶達減少；其中模型Ⅱ組IL-17的mRNA錶達與模型Ⅰ組相比有所降低。對照組和CIA模型Ⅰ組及模型Ⅱ組的小鼠淋巴結中存在ROR-γt和Foxp3分彆與TH、VMAT-2和MAO的共定位細胞。CIA模型組小鼠淋巴結中ROR-γt/TH、ROR-γt/VMAT-2和ROR-γt/MAO雙暘性細胞數增加，模型Ⅱ組中的這些雙暘性細胞數與模型Ⅰ組相比有所減少。結論 CIA模型組小鼠淋巴結中Th17細胞閤成CAs的能力增彊，這種Th17細胞來源的CAs的增加可能在CIA的髮生髮展過程有一定的抗炎作用。
목적：탐토효원유도성관절염（CIA）질병발생발전과정중Th17화Treg세포래원적인다분알（CAs）적작용。방법수궤장18지웅성DBA/1소서분위대조조、CIA모형Ⅰ조（35 d）화CIA모형Ⅱ조（55 d）。Ⅱ형효원유제미근부주사제비CIA소서모형。Real-time PCR법검측림파결중Th17세포적특이성전록인자ROR-γt，상관세포인자IL-17화IL-22，Treg세포적특이성전록인자Foxp3，상관세포인자전화생장인자（TGF）-β이급락안산간화매（TH）적mRNA표체。면역형광조직화학염색법관찰림파결중ROR-γt화Foxp3분별여TH、낭포단알전운체-2（VMAT-2）화단알양화매（MAO）적공정위정황。결과여대조조상비，CIA모형Ⅰ조화모형Ⅱ조적소서림파결중ROR-γ、IL-17、TH화IL-22적mRNA표체증가；Foxp3화TGF-β적mRNA표체감소；기중모형Ⅱ조IL-17적mRNA표체여모형Ⅰ조상비유소강저。대조조화CIA모형Ⅰ조급모형Ⅱ조적소서림파결중존재ROR-γt화Foxp3분별여TH、VMAT-2화MAO적공정위세포。CIA모형조소서림파결중ROR-γt/TH、ROR-γt/VMAT-2화ROR-γt/MAO쌍양성세포수증가，모형Ⅱ조중적저사쌍양성세포수여모형Ⅰ조상비유소감소。결론 CIA모형조소서림파결중Th17세포합성CAs적능력증강，저충Th17세포래원적CAs적증가가능재CIA적발생발전과정유일정적항염작용。
Objective To explore the role of Th17-and Treg-derived catecholamines during collagen-induced ar?thritis (CIA) progression. Methods Eighteen male DBA/1 mice were randomly divided into control group, CIA model groupⅠ(day 35) and CIA model groupⅡ(day 55). The CIA models were induced by typeⅡcollagen (CⅡ) injection from tails. mRNA expression of Th17 specific transcription factor include ROR-γt, cytokines, IL-17 IL-22, Treg specific transcription factor, Foxp3, cytokines, TGF-βand tyrosine hydroxylase (TH) in lymph nodes were examined by real-time PCR. Co-local?ization of ROR-γt or Foxp3, with TH, vesicular monoamine transporter-2 (VMAT-2) or monoamine oxidase (MAO) in lymph nodes were observed by immunofluorescence staining. Results In lymph nodes of mice in CIAⅠgroup and CIAⅡgroup, mRNA expression of ROR-γt, IL-17, TH and IL-22 were upregulated, while mRNA expression of Foxp3 and TGF-βex?pression was downregulated compared to those expression in control group. The upregulated expression of IL-17 was signifi?cantly reduced in CIAⅡgroup compared with that in CIAⅠgroup. In the lymph nodes of both intact and CIA mice, co-lo?calization of ROR-γt or Foxp3 with TH, VMAT-2 or MAO was seen in some cells. The numbers of cells that are double-pos?itive of ROR-γt/TH,ROR-γt/VMAT-2 and ROR-γt/MAO IL-17 were increased in CIA groups compared to those in con?trol group. And they are significantly reduced in CIAⅡgroup compared with those in CIAⅠgroup. Conclusion The abili?ty to synthesize catecholamines in Th17 cells was increased in lymph node in mice from CIA groups compared to that in con?trol group. The increased catecholamines production from Th17 cells in lymph nodes may be involved in the anti-inflammato?ry progression in CIA.
