CAJ | 학술논문

目的：探讨铯137（137 Cs）辐照对库存红细胞表面分子红细胞1型补体受体（complement receptor type 1，CR1 or CD35）及 CD47分子的影响。方法采集60例健康献血者静脉血，制备去白细胞的悬浮红细胞，并将其分为2组各30例，未辐照组常规4℃保存，辐照组采用25Gy 137 Cs辐照后4℃保存。分别于第0、7、14、28、35天收集2mL 样本。采用直标法流式细胞术检测红细胞表面分子 CD35、CD47。结果辐照组和未辐照组红细胞 CD35、CD47的表达均随着4℃保存时间的延长而减少，2组的时点间以及组间·时点间交互作用比较差异均有统计学意义（P ＜0．01），但组间差异无统计学意义（P ＞0．05）。结论25Gy 137 Csγ射线辐照对保存期内红细胞 CD35、CD47表面分子表达无明显影响。
목적：탐토색137（137 Cs）복조대고존홍세포표면분자홍세포1형보체수체（complement receptor type 1，CR1 or CD35）급 CD47분자적영향。방법채집60례건강헌혈자정맥혈，제비거백세포적현부홍세포，병장기분위2조각30례，미복조조상규4℃보존，복조조채용25Gy 137 Cs복조후4℃보존。분별우제0、7、14、28、35천수집2mL 양본。채용직표법류식세포술검측홍세포표면분자 CD35、CD47。결과복조조화미복조조홍세포 CD35、CD47적표체균수착4℃보존시간적연장이감소，2조적시점간이급조간·시점간교호작용비교차이균유통계학의의（P ＜0．01），단조간차이무통계학의의（P ＞0．05）。결론25Gy 137 Csγ사선복조대보존기내홍세포 CD35、CD47표면분자표체무명현영향。
ABSTRACT:Objective To investigate the effects of 25Gy 137 Cs radiation on the red cell surface molecule complement receptor type 1(CD35)or CD35 and CD47 in storage erythrocytes. Methods The vein blood samples of healthy donors were collected,leukocyte-depleted suspended erythrocytes were prepared.The specimens of 60 cases were randomly divided into two groups with 30 cases in each group and stored at 4℃,namely non-irradiation group (control group)and irradiation group.The suspended erythrocytes (2mL)were respectively collected on the 0,7th, 14th,21st,28th,and 35th day after the storage of two groups.The quantity of CD35 and CD47 of erythrocytes were assessed by direct calibration method using flow cytometry.Results The quantity of erythrocytes CD35 and CD47 of non-irradiated group and irradiation group decreased during storage at 4℃.The difference had statistical significance in different storage times and interactions between groups and time (P < 0.01 ).However,intergroup difference was of no significance (P >0.05).Conclusion 25Gy 137 Cs radiation does not have significant effects on the CD35 and CD47 expression.