国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2014年
12期
1100-1103
,共4页
血管性痴呆%巢蛋白%神经元凋亡%空间记忆能力
血管性癡呆%巢蛋白%神經元凋亡%空間記憶能力
혈관성치태%소단백%신경원조망%공간기억능력
Vascular dementia%Nidogen%Neuronal apoptosis%Spatial memory
目的：研究通窍活血汤对血管性痴呆（VaD）大鼠海马CA1区神经元凋亡及血清巢蛋白（Nestin）表达的影响，探讨通窍活血汤改善VaD大鼠空间记忆能力的作用机理。方法选取40只健康雄性SD大鼠，按随机数字表随机分为空白对照组、假手术组、VaD模型组、中药组、安慰剂组各8只，采用Olsson法建立VaD模型，造模成功后，中药组予以通窍活血汤灌胃，安慰剂组予蒸馏水灌胃。Morris水迷宫实验测试其学习记忆能力，TUNEL法检测海马CA1区神经元凋亡情况，ELISA法检测血清Nestin含量。结果中药组VaD大鼠海马CA1区神经元凋亡指数为（37.01±2.23）%，安慰剂组为（55.15±1.54）%，差异有统计学意义（P＜0.05）；中药组血清Nestin含量为（4.47±0.31）ng/L，安慰剂组为（3.42±0.43）ng/L，两组比较差异有统计学意义（P＜0.05）。中药组VaD大鼠第3、4、5天逃避潜伏期[（32.07±13.43）s、（21.08±6.45）s、（19.53±7.52）s]较VaD模型组[（49.71±18.16）s、（34.37±7.42）s、（30.68±6.67）]明显缩短（P均＜0.01），穿越平台次数明显增多（5.02±1.34对3.77±1.07）次, t＝3.521，P＝0.001）。结论通窍活血汤能改善VaD大鼠空间记忆能力，可能与增加VaD大鼠血清Nestin表达和减少海马CA1区神经元凋亡相关。
目的：研究通纖活血湯對血管性癡呆（VaD）大鼠海馬CA1區神經元凋亡及血清巢蛋白（Nestin）錶達的影響，探討通纖活血湯改善VaD大鼠空間記憶能力的作用機理。方法選取40隻健康雄性SD大鼠，按隨機數字錶隨機分為空白對照組、假手術組、VaD模型組、中藥組、安慰劑組各8隻，採用Olsson法建立VaD模型，造模成功後，中藥組予以通纖活血湯灌胃，安慰劑組予蒸餾水灌胃。Morris水迷宮實驗測試其學習記憶能力，TUNEL法檢測海馬CA1區神經元凋亡情況，ELISA法檢測血清Nestin含量。結果中藥組VaD大鼠海馬CA1區神經元凋亡指數為（37.01±2.23）%，安慰劑組為（55.15±1.54）%，差異有統計學意義（P＜0.05）；中藥組血清Nestin含量為（4.47±0.31）ng/L，安慰劑組為（3.42±0.43）ng/L，兩組比較差異有統計學意義（P＜0.05）。中藥組VaD大鼠第3、4、5天逃避潛伏期[（32.07±13.43）s、（21.08±6.45）s、（19.53±7.52）s]較VaD模型組[（49.71±18.16）s、（34.37±7.42）s、（30.68±6.67）]明顯縮短（P均＜0.01），穿越平檯次數明顯增多（5.02±1.34對3.77±1.07）次, t＝3.521，P＝0.001）。結論通纖活血湯能改善VaD大鼠空間記憶能力，可能與增加VaD大鼠血清Nestin錶達和減少海馬CA1區神經元凋亡相關。
목적：연구통규활혈탕대혈관성치태（VaD）대서해마CA1구신경원조망급혈청소단백（Nestin）표체적영향，탐토통규활혈탕개선VaD대서공간기억능력적작용궤리。방법선취40지건강웅성SD대서，안수궤수자표수궤분위공백대조조、가수술조、VaD모형조、중약조、안위제조각8지，채용Olsson법건립VaD모형，조모성공후，중약조여이통규활혈탕관위，안위제조여증류수관위。Morris수미궁실험측시기학습기억능력，TUNEL법검측해마CA1구신경원조망정황，ELISA법검측혈청Nestin함량。결과중약조VaD대서해마CA1구신경원조망지수위（37.01±2.23）%，안위제조위（55.15±1.54）%，차이유통계학의의（P＜0.05）；중약조혈청Nestin함량위（4.47±0.31）ng/L，안위제조위（3.42±0.43）ng/L，량조비교차이유통계학의의（P＜0.05）。중약조VaD대서제3、4、5천도피잠복기[（32.07±13.43）s、（21.08±6.45）s、（19.53±7.52）s]교VaD모형조[（49.71±18.16）s、（34.37±7.42）s、（30.68±6.67）]명현축단（P균＜0.01），천월평태차수명현증다（5.02±1.34대3.77±1.07）차, t＝3.521，P＝0.001）。결론통규활혈탕능개선VaD대서공간기억능력，가능여증가VaD대서혈청Nestin표체화감소해마CA1구신경원조망상관。
Objective To study the effects of Tongqiao-Huoxue decoction on neuron apoptosis in the CA1 region of the hippocampus and the serum nidogen expression of vascular dementia(VaD) rats and to investigate its mechanism. Methods 40 healthy male SD rats were randomly divided into a normal group, a sham operation group, a model group, a TCM group, and a placebo group. VaD rat models were established by Olsson method. After the models were successfully prepared, the rats in the TCM group were fed Tongqiao-Huoxue decoction, the rats in the placebo group were fed distilled water. Morris water maze test was adopted to investigate their learning and memory abilities, TUNEL was used to assay hippocampal CA1 neurons apoptosis, ELISA kits was used to detect Nestin serum levels . Results The neuron apoptosis index in the CA1 region of the hippocampus in the TCM group was(37.01±2.23)%,which was(55.15±1.54)%in the placebo group;the difference was statistically significant(P<0.05).The serum nidogen was(4.47±0.31)ng/L in the TCM group, which was(3.42±0.43)ng/L in the placebo group;the difference was statistically significant(P<0.05). Compared to the VaD group, escape latency in the TCM group in the third, forth, and fifth day was significantly shorter(P<0.05), the number of cross platforms were significantly increased(t=3.521, P=0.000). Conclusion Tong qiao-Huoxue decoction can improve learning and memory impairment in VaD rats, which may be associated with its increasing serum nidogen expression and decreasing the neuron apoptosis in hippocampal CA1.
