CAJ | 학술논문

目的：探讨首乌益智胶囊对脑缺血再灌注大鼠学习记忆和早老素1（PS1）、β淀粉样前体蛋白(APP)mRNA表达的影响。方法80只大鼠按体质量随机分为假手术组、模型组、首乌益智胶囊组、脑复康组，每组20只。采用大脑中动脉闭塞法制作脑缺血再灌模型。造模后7 d，首乌益智胶囊组和脑复康组分别给予首乌益智胶囊溶液(52 mg/ml)、脑复康溶液(28 mg/ml)灌胃，均为1 ml/(100 g?d)，共28 d，模型组和假手术组等体积生理盐水灌胃。采用Morris水迷宫评价学习和记忆；采用实时荧光定量PCR检测大鼠海马PS1和APP mRNA表达。结果水迷宫实验显示，模型组大鼠逃避潜伏期较假手术组[(12.98±0.70)s比(9.43±0.78)s]显著延长，穿越平台次数较假手术组[(5.08±0.39)次比(7.62±0.43)次]显著减少，首乌益智胶囊组逃避潜伏期较模型组[(9.77±0.58)s比(12.98±0.70)s]显著缩短(P均＜0.01)，穿越平台次数较模型组[(7.40±0.44)次比(5.08±0.39)次]显著增加(P均＜0.01)。首乌益智胶囊组海马PS1和APP mRNA 表达[(0.99±0.01)比(1.08±0.03)]均较模型组[(1.06±0.03)比(1.12±0.04)]显著降低(P＜0.05或0.01)。结论首乌益智胶囊可抑制脑缺血再灌大鼠海马PS1和APP mRNA表达，改善学习和记忆。
목적：탐토수오익지효낭대뇌결혈재관주대서학습기억화조로소1（PS1）、β정분양전체단백(APP)mRNA표체적영향。방법80지대서안체질량수궤분위가수술조、모형조、수오익지효낭조、뇌복강조，매조20지。채용대뇌중동맥폐새법제작뇌결혈재관모형。조모후7 d，수오익지효낭조화뇌복강조분별급여수오익지효낭용액(52 mg/ml)、뇌복강용액(28 mg/ml)관위，균위1 ml/(100 g?d)，공28 d，모형조화가수술조등체적생리염수관위。채용Morris수미궁평개학습화기억；채용실시형광정량PCR검측대서해마PS1화APP mRNA표체。결과수미궁실험현시，모형조대서도피잠복기교가수술조[(12.98±0.70)s비(9.43±0.78)s]현저연장，천월평태차수교가수술조[(5.08±0.39)차비(7.62±0.43)차]현저감소，수오익지효낭조도피잠복기교모형조[(9.77±0.58)s비(12.98±0.70)s]현저축단(P균＜0.01)，천월평태차수교모형조[(7.40±0.44)차비(5.08±0.39)차]현저증가(P균＜0.01)。수오익지효낭조해마PS1화APP mRNA 표체[(0.99±0.01)비(1.08±0.03)]균교모형조[(1.06±0.03)비(1.12±0.04)]현저강저(P＜0.05혹0.01)。결론수오익지효낭가억제뇌결혈재관대서해마PS1화APP mRNA표체，개선학습화기억。
Objective To investigate the effects of Shouwu-Yizhi capsule on learning and memory, and the expressions of presenilin 1(PS1),β-amyloid precursor protein (APP) mRNAs in the hippocampus following cerebral ischemia reperfusion in rats. Methods Sprague–Dawley rats were randomly divided into a Shouwu-Yizhi group, a piracetam group, a model group, and a sham operation group with 20 rats in each group. Focal cerebral ischemia reperfusion model was induced by middle cerebral artery occlusion for 2 hours. Seven days after ischemia reperfusion, the rats in the Shouwu-Yizhi and piracetam groups were administered intragastrically Shouwu-Yizhi solution (52 mg/ml) and piracetam solution (28 mg/ml) for 28 days, both in dose of 1 ml/(100 g?d) for 28 days;and the rats in the model and sham operation groups were given intragastrically equivalent volumes of normal saline. Learning and memory were tested using the Morris water maze, and the expressions of PS1 and APP mRNAs were measured using real-time quantitative fluorescent PCR. Results Water maze test showed that the escape latency (12.98±0.70s vs. 9.43±0.78s) was significantly shorter, and the frequency of crossing platform (5.08±0.39 vs. 7.62±0.43) significantly lower in the model groupthan those in the sham operation group; the escape latency (9.77±0.58s vs. 12.98±0.70s) was significantly shorter and the frequency of crossing platform (7.40±0.44 vs. 5.08±0.39) significantly lower in the Shouwu-Yizhi group than those in the model group(all P<0.01). The expressions of PS1 (0.99±0.01 vs. 1.08± 0.03)and APP (1.06±0.03 vs. 1.12±0.04) mRNAs in the hippocampus in the Shouwu-Yizhi group were significantly decreased than those in the model group (P<0.05 or 0.01). Conclusion Shouwu-Yizhi capsule may inhibit the expressions of PS1 and APP mRNAs in the hippocampus, and improve the learning and memory following cerebral ischemia reperfusion in rats.