CAJ | 학술논문

目的：探讨银柴胡提取物对兔脊髓慢性渐进性压迫后基质金属蛋白酶2、12（MMP-2、12）表达及神经功能的影响。方法：家兔随机分为脊髓损伤后应用生理盐水对照组(A组)和银柴胡提取物干预治疗组(B组)，损伤后不同时间取材,HE 染色观察损伤脊髓组织病理变化；免疫组化染色检测MMP-2及Real-Time PCR检测MMP-12的表达变化；采用Tarlov评分评价神经功能。结果：HE染色镜检发现脊髓组织病理学改变B组明显轻于A组。2组均发现MMP-2、12的表达，MMP-2、12表达A 组>B组(P＜0.05)。 B组神经功能改善优于A组。结论：银柴胡提取物可有效抑制家兔脊髓慢性渐进性压迫后MMP-2、12表达，促进神经功能恢复。
목적：탐토은시호제취물대토척수만성점진성압박후기질금속단백매2、12（MMP-2、12）표체급신경공능적영향。방법：가토수궤분위척수손상후응용생리염수대조조(A조)화은시호제취물간예치료조(B조)，손상후불동시간취재,HE 염색관찰손상척수조직병리변화；면역조화염색검측MMP-2급Real-Time PCR검측MMP-12적표체변화；채용Tarlov평분평개신경공능。결과：HE염색경검발현척수조직병이학개변B조명현경우A조。2조균발현MMP-2、12적표체，MMP-2、12표체A 조>B조(P＜0.05)。 B조신경공능개선우우A조。결론：은시호제취물가유효억제가토척수만성점진성압박후MMP-2、12표체，촉진신경공능회복。
Objective:To discuss the influence of YinChaiHu (Radix Stellariae) extracts on neurological func-tion, the expressions of matrix metalloproteinase-2, 12 (MMP-2, 12) of the rabbits suffering from chronic progressive oppressive spinal cord injury. Methods: The rabbits were randomized into the control group (group A) receiving physiological saline and the treatment group (group B) intervened by YinChaiHu extracts after spinal cord injury, the tissue was cut at different times, pathological changes of damaged spinal cord were observed by HE staining;the ex-pressions of MMP-12 by Real-Time PCR and MMP-2 by immuohistochemical staining; neurological function as-sessed by Tarlov scaling. Results:HE staining microscopy revealed that group B was lighter than group A obviously in histopathology of spinal cord. The expressions of MMP-2, 12 were found in both groups, group A was more than group B in the expressions of MMP-2, 12(P<0.05). Group B was superior to group A in the improvements of neuro-logical function. Conclusion:YinChaiHu extracts could effectively inhibit the expressions of MMP-2, 12, promote the recovery of neurological function of the rabbits suffering from chronic progressive oppressive spinal cord injury.