武夷科学
武夷科學
무이과학
WUYI SCIENCE JOURNAL
2014年
1期
195-199
,共5页
孟秀利%李亚%周波%鲁国东
孟秀利%李亞%週波%魯國東
맹수리%리아%주파%로국동
稻瘟病菌%转录因子%侵染初期%筛选
稻瘟病菌%轉錄因子%侵染初期%篩選
도온병균%전록인자%침염초기%사선
Magnaporthe oryzae%transcription factor%earLy stage of infection%screening
稻瘟病是威胁水稻产量的重要病害,弄清楚稻瘟病菌与水稻的互作对于防控稻瘟病具有重要的意义。转录因子是调控基因表达的重要因子,对于调控稻瘟病菌的生长发育和致病性具有重要作用。为了寻找在稻瘟病菌侵染初期特异表达的转录因子,我们首先从稻瘟病菌数据库中找到486个假定的转录因子,然后通过RT-PCR方法筛选出30个在侵染时期特异表达的转录因子。这些转录因子根据结构域的不同可以分为10种类型。具体来讲,9个属于Zn2Cys6类转录因子,4个属于C2H2 zinc finger类转录因子,6个属于HMG类转录因子,携带Transcription factor jumonji和 NucLeic acid-binding,OB-foLd结构域的转录因子分别含有3个,而携带Winged heLix repressor DNA-binding结构域、ssDNA-binding transcriptionaL reguLator结构域、Homeodomain-Like结构域、zinc finger MIZ-type结构域和 zinc finger DHHC-type结构域的转录因子分别含有1个。
稻瘟病是威脅水稻產量的重要病害,弄清楚稻瘟病菌與水稻的互作對于防控稻瘟病具有重要的意義。轉錄因子是調控基因錶達的重要因子,對于調控稻瘟病菌的生長髮育和緻病性具有重要作用。為瞭尋找在稻瘟病菌侵染初期特異錶達的轉錄因子,我們首先從稻瘟病菌數據庫中找到486箇假定的轉錄因子,然後通過RT-PCR方法篩選齣30箇在侵染時期特異錶達的轉錄因子。這些轉錄因子根據結構域的不同可以分為10種類型。具體來講,9箇屬于Zn2Cys6類轉錄因子,4箇屬于C2H2 zinc finger類轉錄因子,6箇屬于HMG類轉錄因子,攜帶Transcription factor jumonji和 NucLeic acid-binding,OB-foLd結構域的轉錄因子分彆含有3箇,而攜帶Winged heLix repressor DNA-binding結構域、ssDNA-binding transcriptionaL reguLator結構域、Homeodomain-Like結構域、zinc finger MIZ-type結構域和 zinc finger DHHC-type結構域的轉錄因子分彆含有1箇。
도온병시위협수도산량적중요병해,롱청초도온병균여수도적호작대우방공도온병구유중요적의의。전록인자시조공기인표체적중요인자,대우조공도온병균적생장발육화치병성구유중요작용。위료심조재도온병균침염초기특이표체적전록인자,아문수선종도온병균수거고중조도486개가정적전록인자,연후통과RT-PCR방법사선출30개재침염시기특이표체적전록인자。저사전록인자근거결구역적불동가이분위10충류형。구체래강,9개속우Zn2Cys6류전록인자,4개속우C2H2 zinc finger류전록인자,6개속우HMG류전록인자,휴대Transcription factor jumonji화 NucLeic acid-binding,OB-foLd결구역적전록인자분별함유3개,이휴대Winged heLix repressor DNA-binding결구역、ssDNA-binding transcriptionaL reguLator결구역、Homeodomain-Like결구역、zinc finger MIZ-type결구역화 zinc finger DHHC-type결구역적전록인자분별함유1개。
Rice bLast is an important disease which threatens the product of rice. It is significant to study the interac-tion between rice and Magnaporthe oryzae. Transcription factors( TFs)can pLay an important roLe in reguLating the ex-pression of genes. The purpose of this articLe is to screen TFs which can speciaLLy express in the earLy stage of infec-tion. 486 TFs were seLected and studied from the genome database of Magnaporthe oryzae and 30 TFs of them were found speciaLLy expressed in the earLy stage of infection by RT-PCR. The 30 TFs were divided into 10 types based on their protein domains. 9 TFs contain Zn2Cys6 domain and 4 TFs contain C2H2 zinc finger domain. 6 TFs contain HMG domain. 3 TFs contain Winged heLix repressor DNA-binding domain or NucLeic acid-binding OB-foLd domain. There are onLy one TF that contains Winged heLix repressor DNA-binding domain,ssDNA-binding transcriptionaL reg-uLator domain,Homeodomain-Like domain,zinc finger MIZ-type domain or zinc finger DHHC-type domain.