生物学杂志
生物學雜誌
생물학잡지
JOURNAL OF BIOLOGY
2014年
6期
60-63,78
,共5页
Aspergillus niger%异淀粉酶%Plackett-Burman试验%响应面分析
Aspergillus niger%異澱粉酶%Plackett-Burman試驗%響應麵分析
Aspergillus niger%이정분매%Plackett-Burman시험%향응면분석
Aspergillus niger%isoamylase%Plackett-Burman experiment%response surface methodology
在液态发酵条件下，采用单因素实验确定了Aspergillus niger PZ331产异淀粉酶的最适碳源和氮源，分别为蔗糖和硝酸铵。在上述基础上利用Plackett-Burman设计对影响产异淀粉酶的因素进行评价，并筛选出硝酸铵、接种量、培养温度3个主要因素；继而利用响应面设计优化了最佳硝酸铵浓度、接种量和培养温度。最终确定了最优培养条件为：蔗糖10 g／L，硝酸铵10 g／L，磷酸氢二钾3 g／L，硫酸亚铁0.01 g／L，硫酸镁1 g／L，起始pH值4.2；接种量2％（孢子浓度为107cfu／mL），30℃培养72 h，酶活达137.3μ／mL；比基础培养基的提高了1.71倍左右。
在液態髮酵條件下，採用單因素實驗確定瞭Aspergillus niger PZ331產異澱粉酶的最適碳源和氮源，分彆為蔗糖和硝痠銨。在上述基礎上利用Plackett-Burman設計對影響產異澱粉酶的因素進行評價，併篩選齣硝痠銨、接種量、培養溫度3箇主要因素；繼而利用響應麵設計優化瞭最佳硝痠銨濃度、接種量和培養溫度。最終確定瞭最優培養條件為：蔗糖10 g／L，硝痠銨10 g／L，燐痠氫二鉀3 g／L，硫痠亞鐵0.01 g／L，硫痠鎂1 g／L，起始pH值4.2；接種量2％（孢子濃度為107cfu／mL），30℃培養72 h，酶活達137.3μ／mL；比基礎培養基的提高瞭1.71倍左右。
재액태발효조건하，채용단인소실험학정료Aspergillus niger PZ331산이정분매적최괄탄원화담원，분별위자당화초산안。재상술기출상이용Plackett-Burman설계대영향산이정분매적인소진행평개，병사선출초산안、접충량、배양온도3개주요인소；계이이용향응면설계우화료최가초산안농도、접충량화배양온도。최종학정료최우배양조건위：자당10 g／L，초산안10 g／L，린산경이갑3 g／L，류산아철0.01 g／L，류산미1 g／L，기시pH치4.2；접충량2％（포자농도위107cfu／mL），30℃배양72 h，매활체137.3μ／mL；비기출배양기적제고료1.71배좌우。
Based on liquid fermentation , the optimum carbon and nitrogen of isoamylase for Aspergillus niger PZ331 were obtained through the single factor experiment , which were sucrose and NH 4 NO3 , respectively .Some process factors of isoamylase production were evaluated by Plackett-Burrman design, and three significant factors ( NH4 NO3 , inoculation amount and temperature ) were ob-tained.Response surface methodology was further used to optimize the three critical internal factors .The results showed that the opti-mum culture medium(g/L) of isoamylase was composed of 10 NH4NO3, 10 sucrose, 3 K2HPO4,0.01FeSO4,1 MgSO4,initial pH 4.2;Under the above conditions , Aspergillus niger PZ331 was cultured under 30℃for 72 h with inoculation amount of 2%( spore concentra-tions of 107cfu/mL), the maximum yield of isoamylase was 137.3 μ/mL, which increased by 1.71-fold.
