中国药品标准
中國藥品標準
중국약품표준
DRUG STANDARDS OF CHINA
2014年
6期
417-419
,共3页
石淋通片%夏佛塔苷%高效液相色谱法%含量测定
石淋通片%夏彿塔苷%高效液相色譜法%含量測定
석림통편%하불탑감%고효액상색보법%함량측정
Shilintong Tablets%schaftoside%HPLC%content determination
目的:建立测定石淋通片中夏佛塔苷含量的HPLC法。方法:采用Diamonsil C18(4.6 mm ×250 mm,5μm)色谱柱,流动相为乙腈-1%乙酸溶液(13∶87),流量1.0 mL· min-1,检测波长272 nm,柱温30℃。结果:夏佛塔苷进样量在0.4614~1.230μg范围内与峰面积呈良好的线性关系( r=0.9997);平均加样回收率为98.49%,其RSD为0.45%。结论:该方法简便、准确,重复性好,可用于石淋通片质量控制。
目的:建立測定石淋通片中夏彿塔苷含量的HPLC法。方法:採用Diamonsil C18(4.6 mm ×250 mm,5μm)色譜柱,流動相為乙腈-1%乙痠溶液(13∶87),流量1.0 mL· min-1,檢測波長272 nm,柱溫30℃。結果:夏彿塔苷進樣量在0.4614~1.230μg範圍內與峰麵積呈良好的線性關繫( r=0.9997);平均加樣迴收率為98.49%,其RSD為0.45%。結論:該方法簡便、準確,重複性好,可用于石淋通片質量控製。
목적:건립측정석림통편중하불탑감함량적HPLC법。방법:채용Diamonsil C18(4.6 mm ×250 mm,5μm)색보주,류동상위을정-1%을산용액(13∶87),류량1.0 mL· min-1,검측파장272 nm,주온30℃。결과:하불탑감진양량재0.4614~1.230μg범위내여봉면적정량호적선성관계( r=0.9997);평균가양회수솔위98.49%,기RSD위0.45%。결론:해방법간편、준학,중복성호,가용우석림통편질량공제。
Objective:To establish an HPLC method to determine the content of schaftoside in Shilintong Tablets.Methods: A Dia-monsil C18 column (4.6 mm ×250 mm,5μm) was adopted with acetonitrile-1%acetic acid (13∶87) as mobile phase at the flow rate of 1.0 mL· min-1.The detection wavelength was 272 nm and the column temperature was 30 ℃.Results:The calibration curve of schaftoside had a good linearity within 0.4614-1.230 μg ( r=0.9997 ).The average recovery was 98.49% and RSD was 0.45%.Conclusion:The method is simple and accurate, which can be used for the quality control of Shilintong Tablets.