CAJ | 학술논문

以苏姜猪、二元杂交猪（长白猪×苏姜猪、大白猪×苏姜猪）为研究对象，分别为60头，分析了HSL基因外显子Ⅰ区域PCR-RFLP多态性。结果表明，苏姜猪及其杂交后代群体中HSL基因外显子I中存在Bsa H I酶切片段长度多态性，均检测到AA、AB和BB基因型。苏姜猪群体中A等位基因占优势，频率为73.34％；杂交猪中B等位基因占优势，频率分别为56.66％和65.00％。研究结果丰富了苏姜猪的分子生物学研究，为苏姜猪的合理开发利用提供了依据。
이소강저、이원잡교저（장백저×소강저、대백저×소강저）위연구대상，분별위60두，분석료HSL기인외현자Ⅰ구역PCR-RFLP다태성。결과표명，소강저급기잡교후대군체중HSL기인외현자I중존재Bsa H I매절편단장도다태성，균검측도AA、AB화BB기인형。소강저군체중A등위기인점우세，빈솔위73.34％；잡교저중B등위기인점우세，빈솔분별위56.66％화65.00％。연구결과봉부료소강저적분자생물학연구，위소강저적합리개발이용제공료의거。
The polymorphism of HSL gene in swine was studied using PCR-RFLP.Total 180 pigs were selected in this experiment, which were Sujiang pig and it′s crossbreed with Large White and Landrace, 60 in each grow.The polymorphism of Bsa H I in HSL gene exon I was detected by restriction fragment length polymorphism (RFLP) method.Three genotypes of AA, AB and BB were de-tected in three pig populations. It showed that Aallele was dominant in Sujiang pig groups with 73.34%, and B allele was detected mainly from the crossbreed with 56.66%and 65.00%, respectively. The results will enrich Sujiang pig molecular biology research, and provide the basis for the reasonable development and application of Sujiang pig.