生物加工过程
生物加工過程
생물가공과정
CHINESE JOURNAL OF BIOPROCESS ENGINEERING
2014年
6期
73-77,95
,共6页
吕翠翠%袁敏%石服鑫%曹慧%徐斐%于劲松
呂翠翠%袁敏%石服鑫%曹慧%徐斐%于勁鬆
려취취%원민%석복흠%조혜%서비%우경송
分子印迹%固相萃取%模板洗脱%超声波辅助抽提%孔雀石绿
分子印跡%固相萃取%模闆洗脫%超聲波輔助抽提%孔雀石綠
분자인적%고상췌취%모판세탈%초성파보조추제%공작석록
molecular imprinting%solid phase extraction%template removal%ultrasound-assisted extraction%malachite green
采用沉淀聚合法制备孔雀石绿分子印迹聚合物（ MG MIPs），以洗脱效率及吸附量为指标，考察超声波辅助抽提法对MIPs中MG洗脱效果及吸附性能的影响，通过扫描电镜观察MIPs的表面形态，并对其吸附性能进行研究。结果表明：模板分子MG在超声30 min、超声10次、料液比m（MG MIPs）∶V（甲醇乙酸溶液）为1∶10（ g／mL）、温度为25℃、超声功率为270 W的条件下，洗脱效果最好，MIPs在固相萃取柱中的吸附效率较高，达到198μg／g。
採用沉澱聚閤法製備孔雀石綠分子印跡聚閤物（ MG MIPs），以洗脫效率及吸附量為指標，攷察超聲波輔助抽提法對MIPs中MG洗脫效果及吸附性能的影響，通過掃描電鏡觀察MIPs的錶麵形態，併對其吸附性能進行研究。結果錶明：模闆分子MG在超聲30 min、超聲10次、料液比m（MG MIPs）∶V（甲醇乙痠溶液）為1∶10（ g／mL）、溫度為25℃、超聲功率為270 W的條件下，洗脫效果最好，MIPs在固相萃取柱中的吸附效率較高，達到198μg／g。
채용침정취합법제비공작석록분자인적취합물（ MG MIPs），이세탈효솔급흡부량위지표，고찰초성파보조추제법대MIPs중MG세탈효과급흡부성능적영향，통과소묘전경관찰MIPs적표면형태，병대기흡부성능진행연구。결과표명：모판분자MG재초성30 min、초성10차、료액비m（MG MIPs）∶V（갑순을산용액）위1∶10（ g／mL）、온도위25℃、초성공솔위270 W적조건하，세탈효과최호，MIPs재고상췌취주중적흡부효솔교고，체도198μg／g。
Malachite green( MG)?molecularly imprinted polymers( MIPs) were prepared via precipitation polymerization?Using elution efficiency and adsorption of malachite green as indexes, we studied the effects of ultrasonic?assisted extraction on the elution of malachite green and adsorptive property of molecularly imprinted polymers were studied?Surface structure of molecularly imprinted polymers was observed by scanning electron microscope ( SEM ) ?Adsorption properties of molecularly imprinted polymers were studied?Results show that the optimum technical parameters for elution efficiency were 30 mins,10 times,the ratio of MG?MIPs to methanol?acetic acid solvent=1/10(g/mL),25 ℃ and 270 W?The adsorption properties of molecularly imprinted polymers in solid phase extraction column attained 198 μg/g.
