CAJ | 학술논문

ε聚赖氨酸生产菌株Streptomyces albulus PD 1可合成一种新型非蛋白质氨基酸均聚物聚二氨基丙酸，采用离子交换层析和反向色谱，对聚二氨基丙酸的分离纯化进行研究。离子交换层析柱选用DEAE Sepharose Fast Flow填料，50 mmol／L 磷酸盐缓冲液（ pH 7?5）平衡上样，含0?5 mol／L NaCl的磷酸盐缓冲液（ pH 7?5）洗脱，收集洗脱液用分子筛 Sephadex G 25除去磷酸盐缓冲液。然后用 C18反相色谱进一步纯化，流动相为 V （甲醇）／V（0?1％磷酸）＝5／95。经过离子交换层析和反向色谱，纯化得到聚二氨基丙酸纯品，回收率为39?8％，样品纯度达98?4％，为后续的聚二氨基丙酸的深入研究奠定基础。
ε취뢰안산생산균주Streptomyces albulus PD 1가합성일충신형비단백질안기산균취물취이안기병산，채용리자교환층석화반향색보，대취이안기병산적분리순화진행연구。리자교환층석주선용DEAE Sepharose Fast Flow전료，50 mmol／L 린산염완충액（ pH 7?5）평형상양，함0?5 mol／L NaCl적린산염완충액（ pH 7?5）세탈，수집세탈액용분자사 Sephadex G 25제거린산염완충액。연후용 C18반상색보진일보순화，류동상위 V （갑순）／V（0?1％린산）＝5／95。경과리자교환층석화반향색보，순화득도취이안기병산순품，회수솔위39?8％，양품순도체98?4％，위후속적취이안기병산적심입연구전정기출。
The ε?polylysine producing strain Streptomyces albulus PD?1 could synthesize a novel non?proteinic amino acid oligomer:poly ( L?diaminopropionic acid ) ( PDAP )?Ion?exchange and reversed?phase chromatography were used to study the purification conditions of PDAP?In the ion?exchange chromatography purification,DEAE?Sepharose Fast Flow was selected,50 mmol/L phosphate buffer was used as mobile phase and 0?5 mol/L NaCl in the phosphate buffer acted as elution?In the reversed?phase chromatography purification,ratio of methanol to 0?1% phosphoric acid was 5/95 ( V/V) and was used as the mobile phase?Pure PDAP sample was obtained by ion?exchange and reversed?phase chromatography, the recovery rate of the purification was 39?8%,and the purity of the sample was 98.4%.