生物加工过程
生物加工過程
생물가공과정
CHINESE JOURNAL OF BIOPROCESS ENGINEERING
2014年
6期
27-32
,共6页
白蚁%纤维素降解菌%筛选%羧甲基纤维素酶%土白蚁特拉布尔希氏菌
白蟻%纖維素降解菌%篩選%羧甲基纖維素酶%土白蟻特拉佈爾希氏菌
백의%섬유소강해균%사선%최갑기섬유소매%토백의특랍포이희씨균
termite%cellulose-degrading bacteria%isolation%carboxymethyl cellulase%Trabulsiella odontotermitis
从黑翅土白蚁（ Odontotermes formosanus Shiraki）肠道内筛选具有纤维素降解能力的细菌，并研究其酶活性。结果表明：筛选得到5株菌株，活力较高的菌株 CMC 4被鉴定为土白蚁特拉布尔希氏菌 Z 4（ Trabulsiella odontotermitis ZJSRU 4）。同时对菌株T?odontotermitis ZJSRU 4进行了系统的研究，它具有完整的纤维素酶系统，主要产羧甲基纤维素酶（ CMCase）和β葡萄糖苷酶，滤纸酶的活力较低。在以羧甲基纤维素钠为碳源的培养基中培养36 h，发酵液中CMCase的比酶活达到20?8 U／mL，培养44 h，β糖苷酶的比酶活达到18?2 U／mL。 CMCase和β葡萄糖苷酶作用的pH分别为6?0和6?5，它们作用的最适温度都为40℃。该菌对纤维质原料具有降解能力，具有潜在的应用价值。
從黑翅土白蟻（ Odontotermes formosanus Shiraki）腸道內篩選具有纖維素降解能力的細菌，併研究其酶活性。結果錶明：篩選得到5株菌株，活力較高的菌株 CMC 4被鑒定為土白蟻特拉佈爾希氏菌 Z 4（ Trabulsiella odontotermitis ZJSRU 4）。同時對菌株T?odontotermitis ZJSRU 4進行瞭繫統的研究，它具有完整的纖維素酶繫統，主要產羧甲基纖維素酶（ CMCase）和β葡萄糖苷酶，濾紙酶的活力較低。在以羧甲基纖維素鈉為碳源的培養基中培養36 h，髮酵液中CMCase的比酶活達到20?8 U／mL，培養44 h，β糖苷酶的比酶活達到18?2 U／mL。 CMCase和β葡萄糖苷酶作用的pH分彆為6?0和6?5，它們作用的最適溫度都為40℃。該菌對纖維質原料具有降解能力，具有潛在的應用價值。
종흑시토백의（ Odontotermes formosanus Shiraki）장도내사선구유섬유소강해능력적세균，병연구기매활성。결과표명：사선득도5주균주，활력교고적균주 CMC 4피감정위토백의특랍포이희씨균 Z 4（ Trabulsiella odontotermitis ZJSRU 4）。동시대균주T?odontotermitis ZJSRU 4진행료계통적연구，타구유완정적섬유소매계통，주요산최갑기섬유소매（ CMCase）화β포도당감매，려지매적활력교저。재이최갑기섬유소납위탄원적배양기중배양36 h，발효액중CMCase적비매활체도20?8 U／mL，배양44 h，β당감매적비매활체도18?2 U／mL。 CMCase화β포도당감매작용적pH분별위6?0화6?5，타문작용적최괄온도도위40℃。해균대섬유질원료구유강해능력，구유잠재적응용개치。
Five mesophilic bacteria with cellulose?degrading ability were isolated from the gut of termites ( Odontotermes formosanus Shiraki) . Strain CMC?4 with high cellulose?degrading ability was identified as Trabulsiella odontotermitis ZJSRU?4 according to physiological tests and phylogenetic analysis?High carboxymethyl cellulase( CMCase) and β?glucosidase activity was detected,and filter paper activity was also produced at a low level. When sodium carboxymethyl cellulose was used as carbon source,CMCase activity reached 20?8 U/mL at 36 h and β?glucosidase activity 18?2 U/mL at 44 h. Maximum CMCase activity was at pH 6?5,40℃,while maximumβ?glucosidase activity was at pH 6?0,40℃?This strain has potential industrial application to produce cellulose.
