CAJ | 학술논문

通过考察氯过氧化物酶（ CPO）发酵相关参数的动力学特征，探究酶产量与参数之间的关联性。结果表明：CPO发酵过程中慢速C源麦芽糖比葡萄糖更有利于调控CPO的稳定合成，前者CPO最高比酶活（179?50 U／mL）比后者（135 U／mL）高出44?5 U／mL，而且产酶高峰期延迟1～2 d；发酵过程pH波动与C源消耗速率密切相关，且对CPO合成具有明显的指标性作用。通过生物量曲线及糖消耗曲线与产酶特征对比判断，菌株合成CPO为中期合成类型。副产物黑色素是菌体成熟时期的一种次生代谢物质，与酶的生物合成存在时间上的同步性。控制C源基质和pH对提高CPO稳定化生产具有一定成效。
통과고찰록과양화물매（ CPO）발효상관삼수적동역학특정，탐구매산량여삼수지간적관련성。결과표명：CPO발효과정중만속C원맥아당비포도당경유리우조공CPO적은정합성，전자CPO최고비매활（179?50 U／mL）비후자（135 U／mL）고출44?5 U／mL，이차산매고봉기연지1～2 d；발효과정pH파동여C원소모속솔밀절상관，차대CPO합성구유명현적지표성작용。통과생물량곡선급당소모곡선여산매특정대비판단，균주합성CPO위중기합성류형。부산물흑색소시균체성숙시기적일충차생대사물질，여매적생물합성존재시간상적동보성。공제C원기질화pH대제고CPO은정화생산구유일정성효。
We correlated fermentation parameters of chloroperoxidase ( CPO) fermentation to study the relationship between enzyme yield and the parameters?The experimental results show that maltose, a slow carbon source,is more conducive to control CPO synthesis than glucose in the CPO fermentation process. CPO concentrations reach 179?50 U/mL, when maltose is used, higher than that of glucose ( 135 U/mL) . The fluctuation of pH is closely related to carbon consumption?Based on the pattern of growth,glucose consumption and enzymatic production,CPO synthesis is parallel to growth?The black pigment is a by?product in the secondary metabolism, indicating of the maturation period of the cell,and it is parallel to enzyme synthesis?Carbon source matrix and pH in fermentation have effect on CPO production.