CAJ | 학술논문

采用峰值场强为100 kV·m－1、脉宽80 ms、脉冲频率为1 Hz的极低频高压脉冲电场（ELF－PEF ）处理－0．1 MPa的PEG －6000溶液中萌发的绿豆种子，研究了绿豆在萌发过程中鲜重、自发发光、延迟发光、丙二醛（MDA ）含量和超氧化物歧化酶（SOD ）活性的变化。结果表明，ELF－PEF对PEG胁迫下萌发的绿豆鲜重、自发发光和延迟发光积分强度促进作用分别达到了28．58％、78．35％和40．6％，说明ELF－PEF可以有效促进绿豆种子萌发过程中的生理代谢，缓解干旱胁迫对细胞的伤害作用。ELF－PEF通过提高PEG胁迫下萌发绿豆中SOD活性，降低MDA含量，缓解干旱胁迫对萌发绿豆的氧化损伤，ELF－PEF对细胞毛细吸水和代谢吸水的促进可能是其缓解干旱伤害的原因。
채용봉치장강위100 kV·m－1、맥관80 ms、맥충빈솔위1 Hz적겁저빈고압맥충전장（ELF－PEF ）처리－0．1 MPa적PEG －6000용액중맹발적록두충자，연구료록두재맹발과정중선중、자발발광、연지발광、병이철（MDA ）함량화초양화물기화매（SOD ）활성적변화。결과표명，ELF－PEF대PEG협박하맹발적록두선중、자발발광화연지발광적분강도촉진작용분별체도료28．58％、78．35％화40．6％，설명ELF－PEF가이유효촉진록두충자맹발과정중적생리대사，완해간한협박대세포적상해작용。ELF－PEF통과제고PEG협박하맹발록두중SOD활성，강저MDA함량，완해간한협박대맹발록두적양화손상，ELF－PEF대세포모세흡수화대사흡수적촉진가능시기완해간한상해적원인。
Mung beans soaked in -0 .1MPa PEG solution were treated by extremely low frequency pulsed electric field (ELF-PEF)with peak electric field strength of 100 kV·m-1 ,pulse width of 80 ms ,and pulse frequency of 1 Hz . Traits like fresh weight ,spontaneous luminescence ,delayed luminescence ,malondialdehyde (MDA ) and superoxide dis-mutase (SOD) activity during mung beans germination were investigated .The fresh weight ,spontaneous luminescence and delayed luminescence integral intensity in germinating mung beans were 28 .58% ,78 .35% and 40 .6% ,respec-tively under PEG stress ,indicating that ELF-PEF can accelerate the physiological metabolismand alleviate cells injury under drought stress . Although with increased SOD activity and simultaneously reducing MDA content , the oxidation damage caused by drought stress was alleviated by ELF-PEF in germinating mung beans .The promoting effect of ELF-PEF on capillary water absorption and metabolism water absorption in cells may be the cause of alleviating drought injury .