CAJ | 학술논문

在中性盐（NaCl ）和碱性盐（Na2 CO3）胁迫下，对采自河西走廊黑河中游的黑果枸杞进行种子萌发及幼苗生长试验，测定了发芽率（Gr ）、发芽势（Gv ）、发芽指数（GI ）、活力指数（VI ）和相对盐害率及幼苗的组织含水量、可溶性蛋白质含量、叶绿素含量、电导率、丙二醛含量和POD含量等指标。结果表明，黑果枸杞种子萌发的NaCl、Na2 CO3浓度的临界值分别是50 mmol·L－1和2．5 mmol·L－1，极限值分别是300 mmol·L－1和100 mmol·L－1；在NaCl和Na2 CO3胁迫下，发芽率分别为69．17％和71．67％；幼苗组织含水量分别由对照的88．97％降低到56．17％、70．27％；可溶性蛋白质含量最大值分别为7．09％、7．73％；叶绿素含量分别由对照的1．27 mg·g－1降到0．78 mg·g－1、0．92 mg ·g－1；电导率分别由对照的25．63％增加到64．77％、74．8％；丙二醛含量分别由对照的1．5μmol·g－1增加到6．9、6．5μmol·g－1；POD活性分别由对照的380．4 U·g－1·min－1降低到139．2 U·g－1·min－1、192．7 U·g－1·min－1。黑果枸杞是盐生植物，低浓度的盐促进萌发，高浓度的抑制萌发；碱性盐更适合其生长；黑果枸杞幼苗在盐胁迫下的生理响应及生态适应综合表现出黑果枸杞更适于碱性盐生长。
재중성염（NaCl ）화감성염（Na2 CO3）협박하，대채자하서주랑흑하중유적흑과구기진행충자맹발급유묘생장시험，측정료발아솔（Gr ）、발아세（Gv ）、발아지수（GI ）、활력지수（VI ）화상대염해솔급유묘적조직함수량、가용성단백질함량、협록소함량、전도솔、병이철함량화POD함량등지표。결과표명，흑과구기충자맹발적NaCl、Na2 CO3농도적림계치분별시50 mmol·L－1화2．5 mmol·L－1，겁한치분별시300 mmol·L－1화100 mmol·L－1；재NaCl화Na2 CO3협박하，발아솔분별위69．17％화71．67％；유묘조직함수량분별유대조적88．97％강저도56．17％、70．27％；가용성단백질함량최대치분별위7．09％、7．73％；협록소함량분별유대조적1．27 mg·g－1강도0．78 mg·g－1、0．92 mg ·g－1；전도솔분별유대조적25．63％증가도64．77％、74．8％；병이철함량분별유대조적1．5μmol·g－1증가도6．9、6．5μmol·g－1；POD활성분별유대조적380．4 U·g－1·min－1강저도139．2 U·g－1·min－1、192．7 U·g－1·min－1。흑과구기시염생식물，저농도적염촉진맹발，고농도적억제맹발；감성염경괄합기생장；흑과구기유묘재염협박하적생리향응급생태괄응종합표현출흑과구기경괄우감성염생장。
Germination rate(Gr) ,germination potential(Gv) ,germination index(GI) ,vigor index(VI) ,relative salt injury rate ,tissue water content ,soluble protein content ,chlorophyll content ,conductivity ,MDA and POD contents were determined to investigate the effect of neutral and alkaline salt stresses on germination and seeding growth of Lycium ruthenium Murr seed .The results indicated that minimum requirement of NaCl and Na2 CO3 were 50 mmol·L-1 and 2 .5 mmol · L-1 , and upper limit were 300 mmol · L-1 and 100 mmol · L-1 , respectively . Compared with the control (88 .97% ) , the germination rates were 69 .17% and 71 .67% , water contents of seedling tissue were reduced to 56 .17% and 70 .27% .Maximum of soluble protein contents were 7 .09% and 7 .73% .Chlorophyll contents were re-duced from 1 .27 mg · g-1 to 0 .78 mg · g-1 and 0 .92 mg · g-1 .Conductivity increased from 25 .63% to 64 .77% and 74 .8% .MDA contents increased from 1 .5μmol·g-1 to 6 .9 μmol·g-1 and 6 .5 μmol·g-1 .POD activity were decreased from 380 .4 U·g-1·min-1 to 139 .2 U·g-1·min-1 and 192 .7 U·g-1·min-1 .Being a halophyte ,low concentration salt promoted the germination of Lycium ruthenium Murr .,but high concentration inhibited its germination .The result indicate that saline land which contains a certain concentration of alkaline salt is suitable for the growth of Lycium ruthenium Murr .