CAJ | 학술논문

研究了中低温稀酸预处理对皇竹草化学组成变化、纤维素酶水解得率与总糖得率的影响，并采用扫描电镜（ SEM）对皇竹草纤维结构变化进行了分析。结果表明，随着硫酸浓度的增大、温度的升高和时间的延长，半纤维素含量大幅度降低，且预处理后纤维素酶水解得率也逐渐增大。较好的预处理条件为100 g皇竹草原料，在固液比1：5（ g：mL）条件下，用质量分数4.0％硫酸在温度110℃下，经过8 h 预处理后，纤维素保留率为87.48％，半纤维素水解率为93.68％，所得固体渣经纤维素酶水解72 h后得率为86.3％（纤维素酶用量40 FPIU/g，以纤维素质量计），100 g原料可得到总糖量为54.53 g。预处理后皇竹草纤维表面和细胞壁受到破坏，表面积增大，有利于纤维素酶水解作用的进行。
연구료중저온희산예처리대황죽초화학조성변화、섬유소매수해득솔여총당득솔적영향，병채용소묘전경（ SEM）대황죽초섬유결구변화진행료분석。결과표명，수착류산농도적증대、온도적승고화시간적연장，반섬유소함량대폭도강저，차예처리후섬유소매수해득솔야축점증대。교호적예처리조건위100 g황죽초원료，재고액비1：5（ g：mL）조건하，용질량분수4.0％류산재온도110℃하，경과8 h 예처리후，섬유소보류솔위87.48％，반섬유소수해솔위93.68％，소득고체사경섬유소매수해72 h후득솔위86.3％（섬유소매용량40 FPIU/g，이섬유소질량계），100 g원료가득도총당량위54.53 g。예처리후황죽초섬유표면화세포벽수도파배，표면적증대，유리우섬유소매수해작용적진행。
The effects of dilute acid pretreatment conditions of Pennisetum grass on the composition,celluloses enzymatic hydrolysis yield,and the yield of total sugars in liquids were investigated at medium-low temperature. And the fiber structures of pretreated residues were studied with scanning electron microscope( SEM ). With the increase of sulfuric concentration, temperature,and retention time,the hemicelluloses content declined considerably,and the yield of enzymatic hydrolysis increased gradually. The recovery of cellulose was 87. 48%,the hemicelluloses hydrolysis rate was 93. 68%,the yield of enzymatic hydrolysis was 86. 3%,and the total sugars yield was 54. 53 g/100 g after the pretreatment when the pretreatment conditions were sulfuric acid concentration 4. 0%,temperature 110℃,residence time 8 h,and cellulase dosage 40 FPIU/g cellulose. The results indicated that the fiber surface and cell wall of Pennisetum grass were broken after acid pretreatment,thus the specific surface area and pore volume increased,and it was beneficial to the enzymatic hydrolysis.